Book chapter
Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets
Alcohol, pp.277-294
Methods in Molecular Biology, Humana Press
2008
DOI: 10.1007/978-1-59745-242-7_19
PMID: 18369925
Abstract
Evaluation of the functional responses of T cells is of importance in determining the mechanism(s) of immunodeficiency resulting from chronic alcohol abuse and other conditions that lead to immune dysfunction. Mice that are chronically exposed to 20% (w/v) ethanol in water develop immunodeficiency and have T cells with abnormal activation profiles, reduced total numbers, increased CD4/CD8 ratios, and an increased memory/naïve phenotype ratio. These cells also have abnormal antigen-specific responses after inoculation of the ethanol mice with model infectious organisms. Study of the functional abnormalities of these cells requires a reliable system that can present appropriate activation stimuli in vitro for the generation of polyclonal or antigen-specific responses in enriched or purified T cells, free of the influence of previously ethanol exposed accessory cells. In this chapter, we describe protocols to assess the T cell response to polyclonal stimulation through the T cell receptor and the use of a model infectious disease bacterium, Listeria monocytogenes, that allows evaluation of the T-cell response to specific peptide epitopes of the bacterium after previous inoculation.
Details
- Title: Subtitle
- Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets
- Creators
- Betty M Young - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, USASusan Wiechert - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, USARuth A Coleman - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, USAPrajwal Gurung - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, USARobert T Cook - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Alcohol, pp.277-294
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-59745-242-7_19
- PMID
- 18369925
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 2008
- Academic Unit
- Anatomy and Cell Biology; Infectious Diseases; Pathology; Internal Medicine
- Record Identifier
- 9984094217402771
Metrics
31 Record Views