Book chapter
Primary Human Hepatocyte Culture for HCV Study
Hepatitis C, pp.373-382
Methods in Molecular Biology, Humana Press
2009
DOI: 10.1007/978-1-59745-394-3_28
PMID: 19009276
Abstract
Studies of HCV pathogenesis and antiviral research have been hampered by the lack of adequate cell-culture and small-animal models. The culturing of human primary hepatocytes would greatly facilitate the model development in HCV research. The availability of robust infectious virus, JFH1 (i.e., genotype 2) strain, will further increase the interest in using primary hepatocyte cultures. This cell model system will significantly enhance research in the areas of antiviral research and host–virus interaction, but obtaining pure and viable human primary hepatocytes is not trivial. We have optimized a method of liver perfusion and primary hepatocyte isolation that allows us to establish robust and reliable human primary hepatocyte cultures. Moreover, we have demonstrated that these primary cultures are susceptible to authentic HCV infection in vitro.
Details
- Title: Subtitle
- Primary Human Hepatocyte Culture for HCV Study
- Creators
- Haizhen Zhu - University of Florida HealthJohn Elyar - University of Florida HealthRobin Foss - University of Florida HealthAlan Hemming - University of Florida Health Science CenterEric Hall - CellzDirect IncEdward L. LeCluyse - CellzDirect IncChen Liu - University of Florida Health Science Center
- Resource Type
- Book chapter
- Publication Details
- Hepatitis C, pp.373-382
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-59745-394-3_28
- PMID
- 19009276
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 2009
- Academic Unit
- Surgery
- Record Identifier
- 9984322803902771
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