Procedures for Mucosal Immunization and Analyses of Cellular Immune Response to Candidate HIV Vaccines in Murine and Nonhuman Primate Models

Shailbala Singh; Pramod Nehete; Patrick Hanley; Bharti Nehete; Guojun Yang; Hong He; Scott M Anthony; Kimberly S Schluns; K. Jagannadha Sastry

doi:10.1007/978-1-4939-1115-8_24

Book chapter

Procedures for Mucosal Immunization and Analyses of Cellular Immune Response to Candidate HIV Vaccines in Murine and Nonhuman Primate Models

Shailbala Singh, Pramod Nehete, Patrick Hanley, Bharti Nehete, Guojun Yang, Hong He, Scott M Anthony, Kimberly S Schluns and K. Jagannadha Sastry

Immunoinformatics, pp.417-455

Methods in Molecular Biology, Springer New York

06/27/2014

DOI: 10.1007/978-1-4939-1115-8_24

PMID: 25048139

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Abstract

Sampling the mucosal tissues and analyses of immune responses are integral to vaccine-development strategies against human immunodeficiency virus (HIV), which is transmitted predominantly across the oro-genital mucosa. While immune assay development and standardization attempts employ mouse models, immunogenicity and protective efficacy that can be extrapolated to humans are realized only from experiments in nonhuman primates. Here, we describe commonly used practices for immunizations in rhesus macaques (Macaca mulatta) along with procedures for obtaining important mucosal tissues samples from macaques and mice. We also describe detailed protocols for two important assays applicable in mouse as well as primate experiments for determining antigen-specific T cells responses induced after vaccination.

Animal Models

Flow Cytometry

Vaccines

Cytokine

ELISPOT

HIV–AIDS

Mucosal immunity

Nonhuman primates

Rhesus macaques

T cells

Details

Title: Subtitle: Procedures for Mucosal Immunization and Analyses of Cellular Immune Response to Candidate HIV Vaccines in Murine and Nonhuman Primate Models
Creators: Shailbala Singh - The University of Texas at Austin
Pramod Nehete - The University of Texas at Austin
Patrick Hanley - The University of Texas at Austin
Bharti Nehete - The University of Texas at Austin
Guojun Yang - The University of Texas at Austin
Hong He - The University of Texas at Austin
Scott M Anthony - The University of Texas at Austin
Kimberly S Schluns - The University of Texas at Austin
K. Jagannadha Sastry - The University of Texas at Austin
Resource Type: Book chapter
Publication Details: Immunoinformatics, pp.417-455
Publisher: Springer New York; New York, NY
Series: Methods in Molecular Biology
DOI: 10.1007/978-1-4939-1115-8_24
PMID: 25048139
eISSN: 1940-6029
ISSN: 1064-3745
Language: English
Date published: 06/27/2014
Academic Unit: Pathology
Record Identifier: 9984183984102771

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