Book chapter
Protocol for the Generation and 3D Culture of Fluorescently Labeled Multicellular Spheroids
Methods in molecular biology (Clifton, N.J.)
01/17/2025
DOI: 10.1007/7651_2024_592
PMID: 39821808
Abstract
Spheroid culture systems have been extensively used to model the three-dimensional (3D) behavior of cells in vitro. Traditionally, spheroids consist of a single cell type, limiting their ability to fully recapitulate the complex inter-cellular interactions observed in vivo. Here we describe a protocol for generating cocultured spheroids composed of two distinct cell types, embedded within a 3D extracellular matrix (ECM) to better study cellular interactions. Fluorescent labeling of each cell type enables clear distinction and visualization, facilitating the analysis of cell invasion, proliferation, and behavior within the matrix. This method is particularly suited for studying matrix invasion, an essential process in cancer metastasis, using both fixed and live cell microscopy. The protocol is versatile and can be adapted for various cell types, providing a robust platform for investigating cell-cell interactions in cancer research, tissue remodeling, and drug screening.
Details
- Title: Subtitle
- Protocol for the Generation and 3D Culture of Fluorescently Labeled Multicellular Spheroids
- Creators
- Emily C Liu - University of IowaAmy L Ryan - University of IowaSinem Koc-Günel - University Hospital Frankfurt
- Resource Type
- Book chapter
- Publication Details
- Methods in molecular biology (Clifton, N.J.)
- DOI
- 10.1007/7651_2024_592
- PMID
- 39821808
- NLM abbreviation
- Methods Mol Biol
- ISSN
- 1940-6029
- eISSN
- 1940-6029
- Language
- English
- Electronic publication date
- 01/17/2025
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984774235402771
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