Book chapter
RNA-Seq Experiment and Data AnalysisData analysis - Chapter 22
Estrogen Receptors, pp.405-424
Methods in Molecular Biology, v. 2418, Springer, Second edition
2022
DOI: 10.1007/978-1-0716-1920-9_22
Abstract
With the ability to obtain several millions of reads per sample, high-throughput RNA sequencing (RNA-Seq) enables investigation of any transcriptome at a fine resolution. Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncRNA, pre-mRNA) can also be investigated using RNA-Seq. While facilitating accurate quantification of gene expression, RNA-Seq offers the opportunity to estimate abundance of isoforms and find novel transcripts and allele-specific transcripts. In this chapter, we describe a protocol to construct an RNA-Seq library for sequencing on Illumina NGS platforms and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of differential gene expression in control versus 17β-estradiol treatment of in vivo or in vitro systems.
Details
- Title: Subtitle
- RNA-Seq Experiment and Data AnalysisData analysis - Chapter 22
- Creators
- Miyuraj Harishchandra Hikkaduwa Withanage - University of IowaHanquan Liang - Southwestern Medical CenterErliang Zeng - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Estrogen Receptors, pp.405-424
- Edition
- Second edition
- Series
- Methods in Molecular Biology; v. 2418
- DOI
- 10.1007/978-1-0716-1920-9_22
- ISSN
- 1064-3745
- Publisher
- Springer; Boston
- Language
- English
- Date published
- 2022
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
- Record Identifier
- 9984224959702771
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