Book chapter
Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide
RNA Interference Techniques, pp.19-37
Neuromethods, Humana Press
04/02/2011
DOI: 10.1007/978-1-61779-114-7_2
Abstract
MicroRNAs (miRNAs) have emerged as important modulators of eukaryotic gene expression through a process called RNA interference (RNAi). Over the last several years, a large amount of work has focused on understanding how miRNAs are expressed and processed to a biologically functional form. This knowledge has enabled the development of RNAi as a molecular tool for investigating basic biological questions or as a therapeutic technique. Artificial miRNA shuttle vectors can be engineered to mimic natural miRNAs and subsequently used to suppress any gene of interest. Here, we describe a simple method to build and functionally validate artificial miRNA shuttles.
Details
- Title: Subtitle
- Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide
- Creators
- Ryan L BoudreauSara E Garwick-CoppensJian LiuLindsay M WallaceScott Q Harper - Department of Pediatrics and Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University College of Medicine, Columbus, USA
- Resource Type
- Book chapter
- Publication Details
- RNA Interference Techniques, pp.19-37
- Publisher
- Humana Press; Totowa, NJ
- Series
- Neuromethods
- DOI
- 10.1007/978-1-61779-114-7_2
- eISSN
- 1940-6045
- ISSN
- 0893-2336
- Language
- English
- Date published
- 04/02/2011
- Academic Unit
- Iowa Neuroscience Institute; Internal Medicine; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984070649702771
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