Book chapter
Redox control of cell cycle-coupled topoisomerase iiα gene expression
Methods in Enzymology, pp.448-459
Elsevier Science & Technology
2002
DOI: 10.1016/S0076-6879(02)53068-8
PMID: 12078518
Abstract
This chapter aims to provide a useful description of methods for studying cell cycle-coupled Topo II gene expression and the possible regulatory role of the intracellular redox state in the processes. A growing body of literature suggests the importance of the intracellular redox state as the possible physiological regulator of cell proliferation. Although the molecular mechanisms are currently not fully understood, it is possible that at least some of the mechanisms could be at the level of cell cycle-coupled gene expression. The assays described in the chapter provide methods to study alterations in gene expression during the cell cycle and possible effects of alterations in the intracellular redox state on cell cycle-coupled variations in gene expression. Although the assays were optimized for the study of Topo II expression, similar approaches can be used to study the redox regulation of other cell cycle-coupled genes.
Details
- Title: Subtitle
- Redox control of cell cycle-coupled topoisomerase iiα gene expression
- Creators
- Prabhat C GoswamiRyuji HigashikuboDouglas R Spitz
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.448-459
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(02)53068-8
- PMID
- 12078518
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 2002
- Academic Unit
- Pathology; Radiation Oncology
- Record Identifier
- 9984047627902771
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