Book chapter
Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology: Assessing Mitochondria
Mitochondrial Medicine Volume 2: Assessing Mitochondria, pp.271-283
Methods in Molecular Biology, v. 2276, Humana Press Inc, Second edition
2021
DOI: 10.1007/978-1-0716-1266-8_21
PMCID: PMC10225246
PMID: 34060049
Abstract
Several methods are available to measure ATP production by isolated mitochondria or permeabilized cells but have several limitations, depending upon the particular assay employed. These limitations may include poor sensitivity or specificity, complexity of the method, poor throughput, changes in mitochondrial inner membrane potential as ATP is consumed, and/or inability to simultaneously assess other mitochondrial functional parameters. Here we describe a novel nuclear magnetic resonance (NMR)-based assay that can be carried out with high efficiency in a manner that alleviates the above problems.
Details
- Title: Subtitle
- Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology: Assessing Mitochondria
- Creators
- Liping Yu - University of IowaBrian D. Fink - University of IowaWilliam I. Sivitz - University of Iowa
- Contributors
- Volkmar Weissig (Editor)Marvin Edeas (Editor)
- Resource Type
- Book chapter
- Publication Details
- Mitochondrial Medicine Volume 2: Assessing Mitochondria, pp.271-283
- Edition
- Second edition
- Series
- Methods in Molecular Biology; v. 2276
- DOI
- 10.1007/978-1-0716-1266-8_21
- PMID
- 34060049
- PMCID
- PMC10225246
- NLM abbreviation
- Methods Mol Biol
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Publisher
- Humana Press Inc; New York
- Number of pages
- 13
- Grant note
- Veterans Affairs Medical Research Funds; US Department of Veterans Affairs 5R01HL073166 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Iowa Affiliate Fraternal Order of the Eagles
- Language
- English
- Date published
- 2021
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Medicine Administration; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984627196202771
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