Book chapter
Simultaneous quantification of mitochondrial ATP and ROS production
Mitochondrial medicine. Volume 1, Probing mitochondrial function, pp.149-159
Methods in molecular biology (Clifton, N.J.), v. 1264, Humana
2015
DOI: 10.1007/978-1-4939-2257-4_14
PMID: 25631011
Abstract
Several methods are available to measure ATP production by isolated mitochondria or permeabilized cells but with a number of limitations, depending upon the particular assay employed. These limitations may include poor sensitivity or specificity, complexity of the method, poor throughput, changes in mitochondrial inner membrane potential as ATP is consumed, and/or inability to simultaneously assess other mitochondrial functional parameters. Here we describe a novel nuclear magnetic resonance (NMR)-based assay that can be carried out with high efficiency in a manner that alleviates the above problems.
Details
- Title: Subtitle
- Simultaneous quantification of mitochondrial ATP and ROS production
- Creators
- Liping Yu - University of IowaBrian D Fink - University of IowaWilliam I Sivitz - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Mitochondrial medicine. Volume 1, Probing mitochondrial function, pp.149-159
- Publisher
- Humana; New York
- Series
- Methods in molecular biology (Clifton, N.J.); v. 1264
- DOI
- 10.1007/978-1-4939-2257-4_14
- PMID
- 25631011
- eISBN
- 9781493922574; 1493922572
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Grant note
- I01 BX000285 / BLRD VA 5R01HL073166 / NHLBI NIH HHS
- Language
- English
- Date published
- 2015
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Medicine Administration; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984632130702771
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