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Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice
Book chapter   Peer reviewed

Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice

Shane A. Heiney, Shogo Ohmae, Olivia A. Kim and Javier F. Medina
Extracellular Recording Approaches, pp.39-71
Neuromethods, Humana Press Inc
01/01/2018
DOI: 10.1007/978-1-4939-7549-5_3
PMCID: PMC6542276
PMID: 31156292
url
https://www.ncbi.nlm.nih.gov/pmc/articles/6542276View
Open Access

Abstract

This chapter presents a method for performing in vivo single-unit extracellular recordings and optogenetics during an associative, cerebellum-dependent learning task in head-fixed mice. The method uses a cylindrical treadmill system that reduces stress in the mice by allowing them to walk freely, yet it provides enough stability to maintain single-unit isolation of neurons for tens of minutes to hours. Using this system, we have investigated sensorimotor coding in the cerebellum while mice perform learned skilled movements.
Cerebellum Electrophysiology Protocol Classical conditioning Behavior Motor learning

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