Book chapter
Site-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences
Gene Targeting Protocols, Vol.133, pp.111-126
Methods in molecular biology (Clifton, N.J.), 133, Humana Press
2000
DOI: 10.1385/1-59259-215-5:111
PMID: 10561835
Abstract
Adeno-associated virus (AAV) is of the genus Dependovirus and a member of the family Parvoviridae (1). AAV is unique, in that it requires co-infection of a second helper virus (i.e., adenovirus [Ad] or herpesvirus) to undergo productive infection. In the absence of a helper virus, AAV will integrate preferentially into the host chromosome (ch). Targeting of AAV to ch19.13.3 qter locus has been documented at a frequency of 70% or greater (2). Because of AAV’s unique ability to integrate site-specifically, extensive research has been devoted to understanding how AAV integrates, with the hope of creating targeting vectors for gene therapy.
Details
- Title: Subtitle
- Site-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences
- Creators
- S M Young Jr - Gene Therapy Center, University of North Carolina at Chapel Hill, USAW XiaoR J Samulski
- Resource Type
- Book chapter
- Publication Details
- Gene Targeting Protocols, Vol.133, pp.111-126
- Publisher
- Humana Press; United States
- Series
- Methods in molecular biology (Clifton, N.J.); 133
- DOI
- 10.1385/1-59259-215-5:111
- PMID
- 10561835
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2000
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
- Record Identifier
- 9984025322502771
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