Book chapter
THE CONTINUOUS ROTATING ANNULAR ELECTROPHORESIS COLUMN - A NOVEL-APPROACH TO LARGE-SCALE ELECTROPHORESIS
Downstream Processing and Bioseparation, pp.285-303
ACS Symposium Series, 419
1990
DOI: 10.1021/bk-1990-0419.ch015
Abstract
The continuous rotating annular electrophoresis (CRAE) column design is capable of processing relatively large flowrates and can be scaled-up for industrial use. Previous modelling studies indicate that this column design offers considerable flexibility in meeting process objectives through the decoupling of several important design parameters. The column geometry, voltage gradient, residence time, angular velocity, and packing material/size can be adjusted in order to achieve the desired separation while controlling the peak temperature rise in the bed. However, actual specification of the operating parameters requires careful consideration of buoyancy effects, dispersion, and electrophoretic mobilities in order to achieve optimum results. A laboratory scale CRAE column has been constructed to verify these findings. This paper summarizes the work that has been done so far in developing the CRAE column.
Details
- Title: Subtitle
- THE CONTINUOUS ROTATING ANNULAR ELECTROPHORESIS COLUMN - A NOVEL-APPROACH TO LARGE-SCALE ELECTROPHORESIS
- Creators
- R A YOSHISATOR DATTAJ P GOROWICZR A BEARDSLEYG R CARMICHAEL
- Resource Type
- Book chapter
- Publication Details
- Downstream Processing and Bioseparation, pp.285-303
- Series
- ACS Symposium Series; 419
- DOI
- 10.1021/bk-1990-0419.ch015
- ISSN
- 1947-5918
- Language
- English
- Date published
- 1990
- Academic Unit
- Nursing; Chemical and Biochemical Engineering; Civil and Environmental Engineering
- Record Identifier
- 9984232159702771
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