Book chapter
Targeted integration by adeno-associated virus
Viral Vectors for Gene Therapy: Methods and Protocols, Vol.76, pp.201-219
Methods in molecular medicine, 76, Humana Press
2003
DOI: 10.1385/1-59259-304-6:201
PMID: 12526165
Abstract
The integration of foreign DNA into the genomes of host cells is of fundamental importance for viral oncology, evolution, transgenic organisms, and gene therapy applications. Expression of foreign genes in eukaryotic cells is highly dependent upon the efficiency of integration events and the site of insertion. Integration can sometimes have detrimental effects on the host cell, such as insertional mutagenesis or activation of proto-oncogenes. For gene therapy, it would therefore be attractive to target insertion to innocuous chromosomal sites. Understanding the mechanisms for integration of foreign DNA and target site selection is crucial for approaches where long-term expression from delivered transgenes is required.
Details
- Title: Subtitle
- Targeted integration by adeno-associated virus
- Creators
- Matthew D Weitzman - Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, CA, USASamuel M Young JrToni CathomenRichard Jude Samulski
- Resource Type
- Book chapter
- Publication Details
- Viral Vectors for Gene Therapy: Methods and Protocols, Vol.76, pp.201-219
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods in molecular medicine; 76
- DOI
- 10.1385/1-59259-304-6:201
- PMID
- 12526165
- ISSN
- 1543-1894
- eISSN
- 1940-6037
- Language
- English
- Date published
- 2003
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
- Record Identifier
- 9984025599802771
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