Book chapter
Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis
Meiosis: methods and protocols, Vol.2818, pp.195-212
Methods in molecular biology (Clifton, N.J.), v. 2818, Springer
2024
DOI: 10.1007/978-1-0716-3906-1_13
Abstract
The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.
Details
- Title: Subtitle
- Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis
- Creators
- Tara Hicks - University of IowaNicola Silva - Masaryk UniversitySarit Smolikove - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Meiosis: methods and protocols, Vol.2818, pp.195-212
- Publisher
- Springer; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); v. 2818
- DOI
- 10.1007/978-1-0716-3906-1_13
- eISBN
- 9781071639061; 1071639064
- ISSN
- 1940-6029
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2024
- Academic Unit
- Biology
- Record Identifier
- 9984696854702771
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