Book chapter
The Power of Gametophyte Transformation
Current Advances in Fern Research, pp.271-284
Springer International Publishing
03/25/2018
DOI: 10.1007/978-3-319-75103-0_13
Abstract
A simple, reliable, and efficient transgenesis method is essential for the establishment of any model organism. Mosses and liverworts, non-seed plants, have a dominant gametophyte generation. As the haploid generation is preferred for transgenesis, multiple methods have been developed to transform gametophytes of the model species Physcomitrella patens and Marchantia polymorpha, firmly establishing them as models of nonvascular, non-seed plants. Only recently, transgenesis has been made possible in vascular non-seed plants, the ferns. Reliable methods of transforming both the sporophyte and the gametophyte generations have been developed. Although the fern sporophyte generation, as in seed plants, is the dominant generation, the gametophytes are free-living, independent entities. This life cycle offers an opportunity for developing gametophyte transgenesis methods. Simple, reliable, and efficient methods for transient and stable Agrobacterium-mediated transformation of gametophytes have been developed for the diploid fern Ceratopteris richardii, long-promoted as a model fern. The ability to transform the gametophyte has greatly facilitated the study of candidate genes functioning in promoting apogamy—a form of asexual alternation of generations. It is our great hope that this method will stimulate further development of C. richardii into a model organism. It is also our hope that this method be adapted for transforming other fern species.
Details
- Title: Subtitle
- The Power of Gametophyte Transformation
- Creators
- Linh Thuy Bui - Department of Biology, Indiana University, Bloomington, USAHolly Long - Department of Biology, The University of Iowa, Iowa City, USAErin E Irish - Department of Biology, The University of Iowa, Iowa City, USAAngela R Cordle - Department of Biology, The University of Iowa, Iowa City, USAChi-Lien Cheng - Department of Biology, The University of Iowa, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Current Advances in Fern Research, pp.271-284
- DOI
- 10.1007/978-3-319-75103-0_13
- Publisher
- Springer International Publishing; Cham
- Language
- English
- Date published
- 03/25/2018
- Academic Unit
- Biology
- Record Identifier
- 9984217421602771
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