Book chapter
Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination
Malaria, pp.493-504
Methods in Molecular Biology, Humana Press
08/25/2012
DOI: 10.1007/978-1-62703-026-7_34
PMID: 22990800
Abstract
CD8 T cells are critical mediators of protection against Plasmodium liver-stage infection. Most studies of the CD8 T cell response to whole parasite Plasmodium vaccines address a single T cell epitope in BALB/c mice, and thus provide limited information. Here, we describe a surrogate activation marker approach that uses the coordinate downregulation of the CD8α chain and upregulation of the integrin CD11a to track the total CD8 T cell response to Plasmodium vaccination via flow cytometry. With this approach, quantitative (magnitude, kinetics) and qualitative (distribution, phenotype, and function) features of the total CD8 T cell response to vaccination with attenuated Plasmodium or other pathogens can be studied.
Details
- Title: Subtitle
- Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination
- Creators
- Katherine L Doll - Department of Microbiology, University of Iowa, Iowa City, USANoah S Butler - Department of Microbiology, University of Iowa, Iowa City, USAJohn T Harty - Department of Microbiology, Pathology and Immunology, University of Iowa, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Malaria, pp.493-504
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-62703-026-7_34
- PMID
- 22990800
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 08/25/2012
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984001129202771
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