Transgenic shuttle vector assays for assessing oxidative B-cell mutagenesis in vivo

K Felix; K Kelliher; G W Bornkamm; S Janz

doi:10.1007/978-3-642-60162-0_45

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Transgenic shuttle vector assays for assessing oxidative B-cell mutagenesis in vivo

Book chapter

Peer reviewed

Transgenic shuttle vector assays for assessing oxidative B-cell mutagenesis in vivo

K Felix, K Kelliher, G W Bornkamm and S Janz

Mechanisms of B Cell Neoplasia 1998, pp.369-377

Current topics in microbiology and immunology, 246, Springer

1999

DOI: 10.1007/978-3-642-60162-0_45

PMID: 10396077

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Abstract

The recent development of transgenic mutagenicity assays provides new opportunities for evaluating mutagenic processes in vivo. To asses mutant frequencies in tissue B cells, we decided to take advantage of two such assays that utilize the transgenic shuttle vectors, lambda LIZ and pUR288. Our main interest in this research is to test two basic premises of inflammation-induced plasmacytoma development in genetically susceptible BALB/c mice; i.e., the possibility that plasmacytoma precursor cells may become targets of phagocyte-mediated oxidative mutagenesis in situ and the prospect that plasmacytoma susceptibility/resistance genes may contribute to these phenotypes by enhancing/reducing oxidative mutagenesis in B cells. Based on our preliminary experience with the lambda LIZ and pUR288 transgenic in vivo mutagenicity tests, we propose to employ these assays as broadly applicable tools for assessing overall mutagenesis during normal and aberrant (malignant) B-cell development. Furthermore, transgenic shuttle vector assays appear to lend themselves as ideal methods to associate general B-cell mutagenesis with the peculiar, B cell-typical somatic hypermutation processes that target the V(D)J gene segment, the proto-oncogene bcl-6 and perhaps other, still unknown loci.

Mutation

Animals

Oxidation-Reduction

Plasmids - genetics

Plasmacytoma - genetics

Mice

Bacteriophage lambda - genetics

Phagocytes - metabolism

Genetic Vectors

B-Lymphocytes - metabolism

Plasmacytoma - metabolism

Details

Title: Subtitle: Transgenic shuttle vector assays for assessing oxidative B-cell mutagenesis in vivo
Creators: K Felix - Laboratory of Genetics, NCI, NIH, Bethesda, MD, USA
K Kelliher
G W Bornkamm
S Janz
Resource Type: Book chapter
Publication Details: Mechanisms of B Cell Neoplasia 1998, pp.369-377
Publisher: Springer; Berlin
Series: Current topics in microbiology and immunology; 246
DOI: 10.1007/978-3-642-60162-0_45
PMID: 10396077
ISSN: 0070-217X
Language: English
Date published: 1999
Academic Unit: Pathology
Record Identifier: 9984083254202771

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