Book chapter
Transgenic shuttle vector assays for determining genetic differences in oxidative B cell mutagenesis in vivo
Methods in Enzymology, pp.434-448
Elsevier Science & Technology
2002
DOI: 10.1016/S0076-6879(02)53067-6
PMID: 12078516
Abstract
Because tumorigenesis is an in vivo phenomenon that can be evaluated optimally only in in vivo studies, it has been postulated that attempts to link tumor development with mutagenesis may be most informative when mutagenesis can also be assessed in vivo. Two transgenic shuttle vector assays have been chosen, XLIZ (Big Blue) and pUR288 (placZ), to explore in mice the role of oxidative B cell mutagenesis in the development of the malignant plasma cell tumor, plasmacytoma (PCT). This chapter provides a brief introduction to the putative role of oxidative stress during mouse plasmacytomagenesis (PCTG) and describes the utility of the kLIZ and pUR288 assays for determining oxidative B cell mutagenesis in vivo. In addition, the chapter introduces a genetic system that employs congenic mouse strains to associate the susceptibility to PCT development with the genetic control of oxidative mutagenesis in the B cell compartment.
Details
- Title: Subtitle
- Transgenic shuttle vector assays for determining genetic differences in oxidative B cell mutagenesis in vivo
- Creators
- Klaus FelixLynne D RockwoodSiegfried Janz
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.434-448
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(02)53067-6
- PMID
- 12078516
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 2002
- Academic Unit
- Pathology
- Record Identifier
- 9984083217502771
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