Vascular occlusion and thrombosis in zebrafish

Pudur Jagadeeswaran; Matthew Cykowski; Bijoy Thattaliyath

doi:10.1016/s0091-679x(04)76022-7

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Vascular occlusion and thrombosis in zebrafish

Book chapter

Peer reviewed

Vascular occlusion and thrombosis in zebrafish

Pudur Jagadeeswaran, Matthew Cykowski and Bijoy Thattaliyath

The Zebrafish: Cellular and Developmental Biology, Vol.76, pp.489-500

76, Methods in cell biology, Academic Press

2004

DOI: 10.1016/s0091-679x(04)76022-7

PMID: 15602889

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Abstract

This chapter discusses the vascular occlusion and thrombosis in zebrafish. The laser-induced vascular injury method is a very reproducible assay to measure thrombus formation using zebrafish larvae because of the advantage that a well-defined target location on the vessel could be easily selected. This is unlike the difficulties in reproducing uniform wounding from one vessel to another in mice that requires the analysis of large numbers of vessels to obtain reliable information. With the vascular occlusion method developed, it is possible to conduct larval screens for endothelium and blood components, such as thrombocytes and other cells, as well as plasma procoagulant, anticoagulant, and fibrinolytic factors along with the factors affecting flow dynamics. The larval vascular occlusion assay presents a powerful tool to discover regulatory elements of blood flow and endothelial cells affecting hemostasis. In addition to the larval screens for thrombus formation, the ability to directly visualize lysis of the thrombus allows the design of the assays for mutations affecting fibrinolysis and wound healing.

Mutation

Thrombosis - physiopathology

Blood Platelets - physiology

Wounds and Injuries - physiopathology

Embolism - physiopathology

Zebrafish - genetics

Blood Vessels - physiopathology

Animals

Cell Adhesion - physiology

Zebrafish - physiology

Blood Coagulation Tests - methods

Thrombosis - genetics

Larva - genetics

Larva - physiology

Microscopy, Fluorescence

Details

Title: Subtitle: Vascular occlusion and thrombosis in zebrafish
Creators: Pudur Jagadeeswaran - Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA
Matthew Cykowski
Bijoy Thattaliyath
Resource Type: Book chapter
Publication Details: The Zebrafish: Cellular and Developmental Biology, Vol.76, pp.489-500
Publisher: Academic Press
Series: 76; Methods in cell biology
DOI: 10.1016/s0091-679x(04)76022-7
PMID: 15602889
ISSN: 0091-679X
Language: English
Date published: 2004
Academic Unit: Cardiology; Stead Family Department of Pediatrics
Record Identifier: 9984093490802771

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