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Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers
Book chapter

Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers

Christopher M Groen and Tina L Tootle
Drosophila Oogenesis, pp.113-124
Methods in Molecular Biology, Springer New York
2015
DOI: 10.1007/978-1-4939-2851-4_8
PMID: 26324433

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Abstract

Visualization of actin cytoskeletal dynamics is critical for understanding the spatial and temporal regulation of actin remodeling. Drosophila oogenesis provides an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in Drosophila egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.
Oogenesis Microscopy Actin cytoskeleton Nurse cells

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