Conference proceeding
Consistent and reproducible positioning in longitudinal imaging for phenotyping genetically modified swine
Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, Vol.9417, pp.941729-941729-7
Proceedings of SPIE
01/01/2015
DOI: 10.1117/12.2081980
Abstract
Recent growth of genetic disease models in swine has presented the opportunity to advance translation of developed imaging protocols, while characterizing the genotype to phenotype relationship. Repeated imaging with multiple clinical modalities provides non-invasive detection, diagnosis, and monitoring of disease to accomplish these goals; however, longitudinal scanning requires repeatable and reproducible positioning of the animals. A modular positioning unit was designed to provide a fixed, stable base for the anesthetized animal through transit and imaging. Post ventilation and sedation, animals were placed supine in the unit and monitored for consistent vitals. Comprehensive imaging was performed with a computed tomography (CT) chest-abdomen-pelvis scan at each screening time point. Longitudinal images were rigidly registered, accounting for rotation, translation, and anisotropic scaling, and the skeleton was isolated using a basic thresholding algorithm. Assessment of alignment was quantified via eleven pairs of corresponding points on the skeleton with the first time point as the reference. Results were obtained with five animals over five screening time points. The developed unit aided in skeletal alignment within an average of 13.13 +/- 6.7 mm for all five subjects providing a strong foundation for developing qualitative and quantitative methods of disease tracking.
Details
- Title: Subtitle
- Consistent and reproducible positioning in longitudinal imaging for phenotyping genetically modified swine
- Creators
- Emily Hammond - University of IowaSamantha K. N. Dilger - University of IowaNicholas Stoyles - University of IowaAlexandra Judisch - University of IowaJohn Morgan - University of IowaJessica C. Sieren - University of Iowa
- Contributors
- B Gimi (Editor)R C Molthen (Editor)
- Resource Type
- Conference proceeding
- Publication Details
- Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, Vol.9417, pp.941729-941729-7
- Publisher
- Spie-Int Soc Optical Engineering
- Series
- Proceedings of SPIE
- DOI
- 10.1117/12.2081980
- ISSN
- 0277-786X
- Number of pages
- 7
- Language
- English
- Date published
- 01/01/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology
- Record Identifier
- 9984318808502771
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