Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains

J.C Selby; M.A Shannon; S.A Boppart

doi:10.1109/MMB.2002.1002308

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Conference proceeding

Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains

J.C Selby, M.A Shannon and S.A Boppart

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578), pp.174-179

2002

DOI: 10.1109/MMB.2002.1002308

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Abstract

A methodology is presented for the design of a microfabricated blister-type device to examine the cellular response of isolated cells and multi-cellular aggregates to finite mechanical strains (1-5%):. The proposed device utilizes microfabrication technologies that incorporate the bonding, transfer, and assembly of thin (3 /spl mu/m < thickness < 100 /spl mu/m) elastic membranes onto rigid substrates with a circular hole or array of circular holes in the formation of diaphragm structures. An analysis of membrane deformations and strain fields of macro- and micro-scale polydimethylsiloxane (PDMS) diaphragm, blister-type devices demonstrates that microfabricated diaphragms can achieve finite strains corresponding to smaller axial displacements than those required for identical strains in macro-scale devices. Smaller axial displacements may decrease the confounding shear loading of the cell culture by the overlying nutrient medium. Additionally, it is shown that the uniform residual stress of a microfabricated diaphragm can be experimentally determined and utilized for more precise control of the incremental finite strains imposed on the diaphragm. Thus, microfabricated blister-type cell straining devices may potentially enhance future quantitative investigations of dynamic mechanically stimulated cell culture.

Industrial Engineering

Design engineering

Aggregates

Cells (biology)

Isolation technology

Educational institutions

Capacitive sensors

Biomembranes

Extracellular

Bonding

Details

Title: Subtitle: Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains
Creators: J.C Selby - Dept. of Mech. & Ind. Eng., Illinois Univ., Urbana, IL, USA
M.A Shannon - Dept. of Mech. & Ind. Eng., Illinois Univ., Urbana, IL, USA
S.A Boppart
Resource Type: Conference proceeding
Publication Details: 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578), pp.174-179
DOI: 10.1109/MMB.2002.1002308
Publisher: IEEE
Language: English
Date published: 2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Dermatology
Record Identifier: 9984025326002771

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