Conference proceeding
Development of a noncontact 3-D fluorescence tomography system for small animal in vivo imaging
Proceedings of SPIE, the international society for optical engineering, Vol.7191(1), pp.nihpa106691-nihpa106691
02/16/2009
DOI: 10.1117/12.808199
PMCID: PMC2706514
PMID: 19587837
Abstract
Fluorescence imaging is an important tool for tracking molecular-targeting probes in preclinical studies. It offers high sensitivity, but nonetheless low spatial resolution compared to other leading imaging methods such CT and MRI. We demonstrate our methodological development in small animal
in vivo
whole-body imaging using fluorescence tomography. We have implemented a noncontact fluid-free fluorescence diffuse optical tomography system that uses a raster-scanned continuous-wave diode laser as the light source and an intensified CCD camera as the photodetector. The specimen is positioned on a motorized rotation stage. Laser scanning, data acquisition, and stage rotation are controlled via LabVIEW applications. The forward problem in the heterogeneous medium is based on a normalized Born method, and the sensitivity function is determined using a Monte Carlo method. The inverse problem (image reconstruction) is performed using a regularized iterative algorithm, in which the cost function is defined as a weighted sum of the L-2 norms of the solution image, the residual error, and the image gradient. The relative weights are adjusted by two independent regularization parameters. Our initial tests of this imaging system were performed with an imaging phantom that consists of a translucent plastic cylinder filled with tissue-simulating liquid and two thin-wall glass tubes containing indocyanine green. The reconstruction is compared to the output of a finite element method-based software package NIRFAST and has produced promising results.
Details
- Title: Subtitle
- Development of a noncontact 3-D fluorescence tomography system for small animal in vivo imaging
- Creators
- Xiaofeng Zhang - Dept. of Radiology, Duke University Medical Center, Box 3302, Durham, NC, 27710Cristian Badea - Dept. of Radiology, Duke University Medical Center, Box 3302, Durham, NC, 27710Mathews Jacob - Dept. of Biomedical Engineering, University of Rochester, Box 639, Rochester, NY, 14627G. Allan Johnson - Dept. of Radiology, Duke University Medical Center, Box 3302, Durham, NC, 27710
- Resource Type
- Conference proceeding
- Publication Details
- Proceedings of SPIE, the international society for optical engineering, Vol.7191(1), pp.nihpa106691-nihpa106691
- DOI
- 10.1117/12.808199
- PMID
- 19587837
- PMCID
- PMC2706514
- NLM abbreviation
- Proc SPIE Int Soc Opt Eng
- ISSN
- 0277-786X
- eISSN
- 1996-756X
- Language
- English
- Date published
- 02/16/2009
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Iowa Neuroscience Institute; Radiation Oncology
- Record Identifier
- 9984070489702771
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