Development of lung tissue phantoms for bioluminescent imaging

Durairaj Kumar; Wenxiang Cong; Frank Bohenkamp; Tarun Kakaday; Peter Taft; Lihong V Wang; Geoffrey McLennan; Eric A Hoffman; Ge Wang

doi:10.1117/12.560530

Conference proceeding

Development of lung tissue phantoms for bioluminescent imaging

Durairaj Kumar, Wenxiang Cong, Frank Bohenkamp, Tarun Kakaday, Peter Taft, Lihong V Wang, Geoffrey McLennan, Eric A Hoffman and Ge Wang

Proceedings of SPIE, Vol.5535(1), pp.687-693

Developments in X-Ray Tomography IV

10/26/2004

DOI: 10.1117/12.560530

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Abstract

White nylon material was chosen to make cylindrical tissue phantoms for development of bioluminescence tomography techniques. A low-level light source, delivered through a optic fiber of core diameter 200mm, was placed at different locations on one phantom surface. The light travels through the phantom, reaches the external surface, and is captured by a liquid nitrogen-cooled CCD camera. The scattering, absorption, and anisotropy parameters of the phantom are obtained by matching the measured light transmission profiles to the profiles generated by the TracePro software. The perturbation analysis, with the homogeneous phantoms, demonstrated that the imaging system is sufficiently sensitive to capture intensity change of higher than 0.5nW/cm2 or a location shift of the light source of more than 200microns. It is observed that the system can distinguish two point light sources with separation of about 2mm. The perturbation analysis is also performed with the heterogeneous phantom. Based on our data, we conclude that there is inherent tomographic information in bioluminescent measures taken on the external surface of the mouse, which suggests the feasibility of bioluminescence tomography for biomedical research using the small animals, especially the mice.

Details

Title: Subtitle: Development of lung tissue phantoms for bioluminescent imaging
Creators: Durairaj Kumar - University of Iowa
Wenxiang Cong - University of Iowa
Frank Bohenkamp - University of Iowa
Tarun Kakaday - University of Iowa
Peter Taft - University of Iowa
Lihong V Wang - Texas A&M Univ. United States
Geoffrey McLennan - University of Iowa
Eric A Hoffman - University of Iowa
Ge Wang - University of Iowa
Resource Type: Conference proceeding
Publication Details: Proceedings of SPIE, Vol.5535(1), pp.687-693
Conference: Developments in X-Ray Tomography IV
DOI: 10.1117/12.560530
ISSN: 0277-786X
eISSN: 1996-756X
Language: English
Date published: 10/26/2004
Academic Unit: Internal Medicine; Radiology; Roy J. Carver Department of Biomedical Engineering
Record Identifier: 9984318692002771

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