Conference proceeding
Direct Numerical Simulation of Micro-Scale Interaction Between Ice and Biological Cells
Advances in Bioengineering, pp.79-87
ASME 2002 International Mechanical Engineering Congress and Exposition, New Orleans, Louisiana, USA, Nov. 17 - 22, 2002
01/01/2002
DOI: 10.1115/IMECE2002-32679
Abstract
The freezing of biological material, such as cells and tissue, duing cryopreservation involves the interaction of ice in the extracellular medium with living cells. This cell-ice interaction critically determines the success of the cryo-preservation protocol, as measured by cell survival and viability after the freeze-thaw process. This paper presents numerical simulations of the response of a cell to freezing. The phase change of the aqueous salt solution outside the cell is computed using a sharp-interface technique. The cell is modeled as a salt solution enclosed by a semi-permeable membrane. We compute the concentration and temperature fields around a single cell in the presence of extracellular ice formation. Parametric variations in the factors affecting the cell-ice interaction are performed to describe the physics of thermo-solutal transport of the interaction. Cell water loss is quantified. The external ice front is computed for both stable and unstable (cellular/dendritic) growth modes. The results show that water egress from the cell is dependent on several controlling parameters in complex ways.
Details
- Title: Subtitle
- Direct Numerical Simulation of Micro-Scale Interaction Between Ice and Biological Cells
- Creators
- L Mao - University of IowaH. S Udaykumar - University of IowaJ. O. M Karlsson - Georgia Institute of Technology
- Resource Type
- Conference proceeding
- Publication Details
- Advances in Bioengineering, pp.79-87
- Conference
- ASME 2002 International Mechanical Engineering Congress and Exposition, New Orleans, Louisiana, USA, Nov. 17 - 22, 2002
- DOI
- 10.1115/IMECE2002-32679
- Publisher
- ASMEDC
- Language
- English
- Date published
- 01/01/2002
- Academic Unit
- Injury Prevention Research Center; Mechanical Engineering
- Record Identifier
- 9984196524502771
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