Conference proceeding
Methods of in-vivo mouse lung micro-CT
Proceedings of SPIE, Vol.5746(1), pp.40-49
Medical Imaging 2005: Physiology, Function, and Structure from Medical Images
04/14/2005
DOI: 10.1117/12.598464
Abstract
Micro-CT will have a profound influence on the accumulation of anatomical and physiological phenotypic changes in natural and transgenetic mouse models. Longitudinal studies will be greatly facilitated, allowing for a more complete and accurate description of events if in-vivo studies are accomplished. The purpose of the ongoing project is to establish a feasible and reproducible setup for in-vivo mouse lung micro-computed tomography ( CT). We seek to use in-vivo respiratory-gated CT to follow mouse models of lung disease with subsequent recovery of the mouse. Methodologies for optimizing scanning parameters and gating for the in-vivo mouse lung are presented. A Scireq flexiVent ventilated the gas-anesthetized mice at 60 breaths/minute, 30 cm H20 PEEP, 30 ml/kg tidal volume and provided a respiratory signal to gate a Skyscan 1076 CT. Physiologic monitoring allowed the control of vital functions and quality of anesthesia, e.g. via ECG monitoring. In contrary to longer exposure times with ex-vivo scans, scan times for in-vivo were reduced using 35 m pixel size, 158ms exposure time and 18 m pixel size, 316ms exposure time to reduce motion artifacts. Gating via spontaneous breathing was also tested. Optimal contrast resolution was achieved at 50kVp, 200 A, applying an aluminum filter (0.5mm). There were minimal non-cardiac related motion artifacts. Both 35 m and 1 m voxel size images were suitable for evaluation of the airway lumen and parenchymal density. Total scan times were 30 and 65 minutes respectively. The mice recovered following scanning protocols. In-vivo lung scanning with recovery of the mouse delivered reasonable image quality for longitudinal studies, e.g. mouse asthma models. After examining 10 mice, we conclude CT is a feasible tool evaluating mouse models of lung pathology in longitudinal studies with increasing anatomic detail available for evaluation as one moves from in-vivo to ex-vivo studies. Further developments include automated bronchial tree segmentation and airway wall thickness measurement tools. Improvements in Hounsfield unit calibration have to be performed when the interest of the study lies in determining and quantifying parenchymal changes and rely on estimating partial volume contributions of underlying structures to voxel densities.
Details
- Title: Subtitle
- Methods of in-vivo mouse lung micro-CT
- Creators
- Wolfgang A Recheis - Univ. of Iowa (USA)Earl Nixon - Univ. of Iowa (USA)Jacqueline Thiesse - Univ. of Iowa (USA)Geoffrey McLennan - Univ. of Iowa (USA)Alan Ross - Univ. of Iowa (USA)Eric Hoffman - Univ. of Iowa (USA)
- Resource Type
- Conference proceeding
- Publication Details
- Proceedings of SPIE, Vol.5746(1), pp.40-49
- Conference
- Medical Imaging 2005: Physiology, Function, and Structure from Medical Images
- DOI
- 10.1117/12.598464
- ISSN
- 0277-786X
- Language
- English
- Date published
- 04/14/2005
- Academic Unit
- Radiation Oncology; Roy J. Carver Department of Biomedical Engineering; Internal Medicine; Radiology; Anesthesia
- Record Identifier
- 9984047783202771
Metrics
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