Conference proceeding
NAD(P)H oxidase-induced oxidative stress in sympathetic ganglia of apolipoprotein E deficient mice
Autonomic neuroscience, Vol.126, pp.285-291
2006
DOI: 10.1016/j.autneu.2006.02.005
PMID: 16584925
Abstract
Superoxide anion (O
2
•−) is increased throughout the arterial wall in atherosclerosis. The oxidative stress contributes to lesion formation and vascular dysfunction. In the present study, we tested the hypothesis that NAD(P)H oxidase-derived O
2
•− is increased in nodose sensory ganglia and sympathetic ganglia of apolipoprotein E deficient (apoE
−/−) mice, an established animal model of atherosclerosis. O
2
•− measured ex vivo by L-012-enhanced chemiluminescence was increased by 79
±
17% in whole sympathetic ganglia from apoE
−/− mice (
n
=
5) compared with sympathetic ganglia from control mice (
n
=
5) (
P
<
0.05). In contrast, O
2
•− was not elevated in nodose ganglia from apoE
−/− mice. Dihydroethidium staining confirmed the selective increase in O
2
•− in sympathetic ganglia of apoE
−/− mice, and revealed the contribution of both neurons and non-neuronal cells to the O
2
•− generation. We investigated the enzymatic source of increased O
2
•− in sympathetic ganglia of apoE
−/− mice. The mRNA expression of gp91phox, p22phox, p67phox, and p47phox subunits of NAD(P)H oxidase measured by real time RT-PCR was increased ∼
3–4 fold in sympathetic ganglia of apoE
−/− mice (
n
=
5) compared with control ganglia (
n
=
5). NADPH oxidase activity measured by lucigenin chemiluminescence was increased by 68
±
12% in homogenates of sympathetic ganglia from apoE
−/− mice (
n
=
7) compared with control ganglia (
n
=
7) (
P
<
0.05). The results identify sympathetic ganglia as a novel site of oxidative stress in atherosclerosis, and suggest that upregulation of NAD(P)H oxidase is the source of increased O
2
•− generation. We speculate that oxidative stress in sympathetic ganglia may contribute to impaired baroreflex control of sympathetic nerve activity.
Details
- Title: Subtitle
- NAD(P)H oxidase-induced oxidative stress in sympathetic ganglia of apolipoprotein E deficient mice
- Creators
- Xiuying MaHannah J ZhangCarol A WhiteisXin TianRobin L DavissonKevin C KregelFrancois M AbboudMark W Chapleau
- Resource Type
- Conference proceeding
- Publication Details
- Autonomic neuroscience, Vol.126, pp.285-291
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.autneu.2006.02.005
- PMID
- 16584925
- ISSN
- 1566-0702
- eISSN
- 1872-7484
- Language
- English
- Date published
- 2006
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Provost Office Administration; Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984025335102771
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