Conference proceeding
Polynomial-time disulfide bond determination using mass spectrometry data
2009 IEEE International Conference on Bioinformatics and Biomedicine Workshop, pp.79-86
11/2009
DOI: 10.1109/BIBMW.2009.5332141
Abstract
We present an algorithmic approach for determining, in polynomial time, disulfide bonds in proteins using mass spectrometry data. The proposed technique is based on matching the set of all theoretically possible disulfide bonded structures with precursor ions derived from a tandem MS/MS experiment. For each match found, theoretical fragments from a disulfide bonded peptide structure are matched with precursor ion fragments obtained from the same tandem MS/MS data set in order to determine a protein's disulfide linkage pattern. A polynomial-time approximation strategy is proposed to selectively generate the set of theoretically possible disulfide bonded linkages for matching. Experiments demonstrate the efficacy of the method.
Details
- Title: Subtitle
- Polynomial-time disulfide bond determination using mass spectrometry data
- Creators
- W. Murad - San Francisco State UniversityR. Singh - San Francisco State UniversityTen-Yang Yen - San Francisco State University
- Resource Type
- Conference proceeding
- Publication Details
- 2009 IEEE International Conference on Bioinformatics and Biomedicine Workshop, pp.79-86
- Publisher
- IEEE
- DOI
- 10.1109/BIBMW.2009.5332141
- Language
- English
- Date published
- 11/2009
- Academic Unit
- Computer Science
- Record Identifier
- 9984446456802771
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