Conference proceeding
Preparative separation, structural analysis and evaluation of dentin stiffening activity of grape seed oligomeric proanthocyanidins
Planta Medica, Vol.80(10)
American Society of Pharmacognosy 2014 Annual Meeting – Held in conjunction with the 14th Annual Oxford International Conference on the Science of Botanicals - Oxford, Mississippi, USA - 2014
07/14/2014
DOI: 10.1055/s-0034-1382673
Abstract
A strong and stable dentin-collagen matrix is a prerequisite for building long lasting dental restorations. From previous studies, a 15-fold increase in the dentin stiffening activity was observed on treatment with crude grape seed extract (GSE). Dentin stiffness assay is performed on demineralized dentin beams using a Shimadzu EZ-graph universal testing machine that evaluates mechanical strength of the dentin 1hr post treatment with sample. A partition of the GSE enriched in PAC oligomers from n = 2 to n = 4 (GSE-MP) was produced using a 3 phase solvent system. GSE-MP was found to be more active than the crude extract. A novel centrifugal partition chromatography (CPC) method using 2 step elution and 1 step extrusion has been developed to separate the complex mixture of grape seed oligomeric PACs. GSE-MP also contains galloylated oligomeric PACS (GOPACs). We have previously observed that galloylation increases the dentin stiffness activity in case of monomeric PACs. Thus isolation of GOPACs from GSE-MP using CPC followed by preparative reverse phase HPLC (RP-HPLC) is targeted in order to perform structural analyses as well as dentin bio-modification studies.
Fig. 1
Details
- Title: Subtitle
- Preparative separation, structural analysis and evaluation of dentin stiffening activity of grape seed oligomeric proanthocyanidins
- Creators
- R S Phansalkar - Department of Medicinal Chemistry and Pharmacognosy, College Of PharmacyS N Chen - Department of Medicinal Chemistry and Pharmacognosy, College Of PharmacyJ Napolitano - Department of Medicinal Chemistry and Pharmacognosy, College Of PharmacyJ W Nam - Department of Medicinal Chemistry and Pharmacognosy, College Of PharmacyJ B Mc Alpine - Department of Medicinal Chemistry and Pharmacognosy, College Of PharmacyT Aguiar - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, IL 60612C Vidal - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, IL 60612A Bedran-Russo - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, IL 60612G F Pauli - Department of Medicinal Chemistry and Pharmacognosy, College Of Pharmacy
- Resource Type
- Conference proceeding
- Publication Details
- Planta Medica, Vol.80(10)
- Conference
- American Society of Pharmacognosy 2014 Annual Meeting – Held in conjunction with the 14th Annual Oxford International Conference on the Science of Botanicals - Oxford, Mississippi, USA - 2014
- DOI
- 10.1055/s-0034-1382673
- ISSN
- 0032-0943
- eISSN
- 1439-0221
- Language
- English
- Date published
- 07/14/2014
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984065802302771
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