Conference proceeding
Quantitating pathogenic biofilm architecture in biopsied tissue
Proceedings of the IEEE VisWeek: Workshop on Visual Analytics in Healthcare: Understanding the Physicians Perspective, October 23rd, 2011, Providence, RI , pp.49-52
10/23/2011
Abstract
Accurate diagnosis and treatment of biofilm infections require identification of the pathogenic organism(s) as well as determining the progress of the disease. Current tools in clinical use, including culturing and PCR tests, are extremely useful for identifying organisms, but are destructive in nature – resulting in the loss of important information regarding biofilm architecture and state. Improving clinical understanding of these, often treatment-resistant, infections is of great importance, and new non-destructive imaging-based tools must be developed in order to gather crucial information regarding disease. Here we present new software, ProkaryMetrics, designed to take advantage of available microscopy imaging modalities, providing a unique platform for 3D imaging and analysis of biofilm samples. We demonstrate the software capabilities by analysis of murine tissue biopsy samples containing uropathogenic Escherichia coli biofilms: wild type UTI89 and UTI89∆kpsF strains. Using ProkaryMetrics, we establish significant architectural differences with qualitative 3D visualizations as well as quantitative measurements including volumetric biofilm size, bacterial counts, community density, orientations, and lengths
Details
- Title: Subtitle
- Quantitating pathogenic biofilm architecture in biopsied tissue
- Creators
- Shareef M Dabdoub - The Ohio State UniversityBrian A. VanderBrinkSheryl S. JusticeWilliam C. Ray
- Resource Type
- Conference proceeding
- Publication Details
- Proceedings of the IEEE VisWeek: Workshop on Visual Analytics in Healthcare: Understanding the Physicians Perspective, October 23rd, 2011, Providence, RI , pp.49-52
- Language
- English
- Date published
- 10/23/2011
- Academic Unit
- Periodontics; Dental Research
- Record Identifier
- 9984530397502771
Metrics
1 Record Views