Conference proceeding
The Effect of Proteolytic Enzymatic Treatment on Plastic Deformation of Porcine Aortic Tissue
Advances in Bioengineering, pp.273-274
ASME 2004 International Mechanical Engineering Congress and Exposition, Anaheim, California, USA, Nov. 13 - 19, 2004
01/01/2004
DOI: 10.1115/IMECE2004-60038
Abstract
We investigated the feasibility of developing an in vitro abdominal aortic aneurysm model by mechanically stretching passive porcine aortic tissue after enzymatic degradation of its structural proteins. Individual arterial rings, taken from the thoracic and abdominal porcine aorta were exposed to specific concentrations of collagenase (to degrade collagen) and/or elastase (to degrade elastin). The specimens were then cut into flat strips and subjected to uniaxial incremental creep tests of increasing load amplitude. The plastic strain (strain at zero load) after each creep load cycle was recorded until specimen failure. The maximum plastic strain due to enzymatic degradation and mechanical loading in elastase treated specimens (mean+SD = 45.1±14.7%; p<0.001) was significantly higher than in untreated controls (19.4±7.61%). However, the plastic strain in collagenase treated specimens (18.4±10.3%; p = 0.79) was not significantly different from the controls. Because AAA is associated with a plastic deformation of 200–400%, the results suggest that it is not feasible to develop an in vitro AAA model by enzymatic degradation and biomechanical stretching of passive porcine aorta. Consequently, these findings suggest that aneurysm growth is unlikely to be solely the result of pressure induced passive stretch of a weakened aorta.
Details
- Title: Subtitle
- The Effect of Proteolytic Enzymatic Treatment on Plastic Deformation of Porcine Aortic Tissue
- Creators
- Jarin Kratzberg - University of IowaPatricia Walker - University of IowaElizabeth Rikkers - University of IowaMadhavan Raghavan - University of Iowa
- Resource Type
- Conference proceeding
- Publication Details
- Advances in Bioengineering, pp.273-274
- Conference
- ASME 2004 International Mechanical Engineering Congress and Exposition, Anaheim, California, USA, Nov. 13 - 19, 2004
- Publisher
- ASMEDC
- DOI
- 10.1115/IMECE2004-60038
- ISSN
- 0360-9960
- Language
- English
- Date published
- 01/01/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984196983802771
Metrics
33 Record Views