Conference proceeding
The dynamics of synapse formation: Time-lapse observations by confocal fluorescence microscopy in hippocampal slices and zebra-fish embryos
Proceedings, annual meeting, Electron Microscopy Society of America, Vol.51, pp.100-101
08/01/1993
DOI: 10.1017/S0424820100146345
Abstract
Our present information about the formation of synapses in the central nervous system is based almost exclusively on observations of fixed tissue specimens. Such observations can only provide very limited inference about the dynamics of the underlying developmental processes. In the hopes of obtaining direct information about the dynamics of synapse formation, we have explored new methods that allow repeated, high-resolution optical imaging of synapse formation events in live neural tissues.Our efforts have focused on specific excitatory synapses in two vertebrate nervous systems: (1) the mossy-fiber-to-CA3-pyramidal-cell synapse in the hippocampus of postnatal day 4-8 rats and (2) the mauthner-fiber-to-motoneuron synapse in intact early embryos of the zebrafish Brachydanio rerio. We are using laser-scanning confocal fluorescence microscopy and fluorescent membrane stains such as di- I and di-O. Individual neurons or limited subsets of the neurons within a tissue are stained by applying these dyes in dimethylformamide solution through micropipettes.
Details
- Title: Subtitle
- The dynamics of synapse formation: Time-lapse observations by confocal fluorescence microscopy in hippocampal slices and zebra-fish embryos
- Creators
- Stephen J SmithMichael E DaileySusan PikeJoAnn BuchananDwight E Bergles
- Resource Type
- Conference proceeding
- Publication Details
- Proceedings, annual meeting, Electron Microscopy Society of America, Vol.51, pp.100-101
- DOI
- 10.1017/S0424820100146345
- ISSN
- 0424-8201
- eISSN
- 2690-1315
- Language
- English
- Date published
- 08/01/1993
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984071682802771
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