Conference proceeding
The role of interleukin-4 in the induction phase of allogeneic neonatal tolerance
Transplantation, Vol.62(12), pp.1847-1854
Annual Meeting of the American Society of Transplant Physicians
1996
DOI: 10.1097/00007890-199612270-00029
PMID: 8990375
Abstract
We previously reported that prolonged graft survival in neonatally tolerant mice was associated with enhanced Th2/Th1 cytokines. To determine whether Th2 CD4 cells function in tolerance, we examined whether we could prevent tolerance by blocking Th2 CD4 maturation, using anti-interleukin(IL)-4 monoclonal antibody treatment during neonatal antigen exposure. Anti-IL-4 treatment restored the ability BALB/c of mice to reject A/J skin grafts and blocked the induction of tolerance through multiple mechanisms. Anti-IL-4 treatment blocked the development of donor microchimerism and recovered the ability of mice to proliferate and to generate appropriate delayed-type hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) responses against A/J in a dose-dependent manner. Low-dose anti-IL-4 recovered DTH responses and interferon (IFN)-γ production, but failed to completely prevent IL-4 production or to recover the CTL activity. No A/J-reactive IFN-γ-producing CD8 cells were detected in these mice. In contrast, mice treated with higher doses of anti-IL-4 generated normal CTL responses against A/J, and contained A/J-reactive IFN-γ-producing CD8 cells. The recovery of CTL responses and IFN-γ-producing CD8 cells was associated with a more complete blocking of Th2 cytokine production. Therefore, the presence of IL-4 may play an important role in the induction of neonatal tolerance by shifting maturation of CD4 cells toward Th2 cells and away from Th1 cells, and also by preventing maturation of alloreactive CD8 CTL cells.
Details
- Title: Subtitle
- The role of interleukin-4 in the induction phase of allogeneic neonatal tolerance
- Creators
- Qinglin Gao - Department of Medicine, University of Iowa College of Medicine, Iowa City, 52242, United StatesNaixi Chen - Department of Medicine, University of Iowa College of Medicine, Iowa City, 52242, United StatesTodd M Rouse - Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246, United StatesElizabeth H Field - Department of Medicine, University of Iowa College of Medicine, Iowa City, 52242, United States
- Resource Type
- Conference proceeding
- Publication Details
- Transplantation, Vol.62(12), pp.1847-1854
- Conference
- Annual Meeting of the American Society of Transplant Physicians
- Publisher
- Lippincott
- DOI
- 10.1097/00007890-199612270-00029
- PMID
- 8990375
- ISSN
- 0041-1337
- eISSN
- 1534-6080
- Grant note
- American Society of Transplant Physicians
- Language
- English
- Date published
- 1996
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094737302771
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