Conference proceeding
The suppression of the synthesis of a nuclear protein in cells blocked in G2 phase : identification of NP-170 as topoisomerase II
Radiation research, Vol.132(2), pp.162-167
1992
DOI: 10.2307/3578522
PMID: 1332112
Abstract
Previous studies of a nuclear protein of molecular weight 170 kDa (NP-170) have shown it to have two interesting properties. First, NP-170 synthesis began in mid- to late S phase and became maximal in G2 phase. Second, the synthesis of NP-170 was suppressed in cells blocked in G2 phase following irradiation with 6.8 Gy (J. M. Holland et al., Radiat. Res. 122, 197-208, 1990). The molecular weight of NP-170 is the same as that of Topoisomerase II (Topo II), an enzyme involved in the alteration of DNA supercoiling status with a double-strand passing function. This study was undertaken to determine whether NP-170 could be Topo II. The results from the present study show that both the proteins have identical cell cycle synthesis patterns. The synthesis of both these proteins is suppressed following irradiation. NP-170 was found to be recognized by a Topo II antibody in both Western blots and immunoprecipitation. This study characterizes NP-170 as Topo II.
Details
- Title: Subtitle
- The suppression of the synthesis of a nuclear protein in cells blocked in G2 phase : identification of NP-170 as topoisomerase II
- Creators
- P. C GOSWAMI - Washington univ. school medicine, radiation oncology cent., sect. cancer biology, Saint Louis MO 63108, United StatesM HILL - Washington univ. school medicine, radiation oncology cent., sect. cancer biology, Saint Louis MO 63108, United StatesR HIGASHIKUBO - Washington univ. school medicine, radiation oncology cent., sect. cancer biology, Saint Louis MO 63108, United StatesW. D WRIGHT - Washington univ. school medicine, radiation oncology cent., sect. cancer biology, Saint Louis MO 63108, United StatesJ. L ROTI ROTI - Washington univ. school medicine, radiation oncology cent., sect. cancer biology, Saint Louis MO 63108, United States
- Resource Type
- Conference proceeding
- Publication Details
- Radiation research, Vol.132(2), pp.162-167
- Publisher
- Radiation Research Society; Oak Brook, Il
- DOI
- 10.2307/3578522
- PMID
- 1332112
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Language
- English
- Date published
- 1992
- Academic Unit
- Oral Pathology, Radiology and Medicine; Dental Clinic Administration; Radiation Oncology
- Record Identifier
- 9984047852502771
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