Conference proceeding
Three-dimensional visual truth of the normal airway tree for use as a quantitative comparison to micro-CT reconstructions
Proceedings of SPIE, Vol.5746(1), pp.50-57
Medical Imaging 2005: Physiology, Function, and Structure from Medical Images
04/14/2005
DOI: 10.1117/12.596806
Abstract
Mouse models are important for pulmonary research to gain insight into structure and function in normal and diseased states, thereby extending knowledge of human disease conditions. The flexibility of human disease induction into mice, due to their similar genome, along with their short gestation cycle makes mouse models highly suitable as investigative tools. Advancements in non-invasive imaging technology, with the development of micro-computed tomography ( -CT), have aided representation of disease states in these small pulmonary system models. The generation of CT 3D airway reconstructions has to date provided a means to examine structural changes associated with disease. The degree of accuracy of CT is uncertain. Consequently, the reliability of quantitative measurements is questionable. We have developed a method of sectioning and imaging the whole mouse lung using the Large Image Microscope Array (LIMA) as the gold standard for comparison. Fixed normal mouse lungs were embedded in agarose and 250 m sections of tissue were removed while the remaining tissue block was imaged with a stereomicroscope. A complete dataset of the mouse lung was acquired in this fashion. Following planar image registration, the airways were manually segmented using an in-house built software program PASS. Amira was then used render the 3D isosurface from the segmentations. The resulting 3D model of the normal mouse airway tree developed from pathology images was then quantitatively assessed and used as the standard to compare the accuracy of structural measurements obtained from -CT.
Details
- Title: Subtitle
- Three-dimensional visual truth of the normal airway tree for use as a quantitative comparison to micro-CT reconstructions
- Creators
- Jacqueline Thiesse - University of IowaJoseph M Reinhardt - University of IowaJessica de Ryk - University of IowaEman Namati - University of IowaJessica Leinen - University of IowaWolfgang A Recheis - University of IowaEric A Hoffman - University of IowaGeoffrey McLennan - University of Iowa
- Resource Type
- Conference proceeding
- Publication Details
- Proceedings of SPIE, Vol.5746(1), pp.50-57
- Conference
- Medical Imaging 2005: Physiology, Function, and Structure from Medical Images
- DOI
- 10.1117/12.596806
- ISSN
- 0277-786X
- Language
- English
- Date published
- 04/14/2005
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Internal Medicine; Radiology
- Record Identifier
- 9984196973502771
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