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Trypsin-Ligand Binding Free Energy Calculation with AMOEBA
Conference proceeding

Trypsin-Ligand Binding Free Energy Calculation with AMOEBA

Yue Shi, Dian Jiao, Michael J Schnieders and Pengyu Ren
Conference proceedings (IEEE Engineering in Medicine and Biology Society. Conf.), Vol.1, pp.2328-2331
2009
DOI: 10.1109/IEMBS.2009.5335108
PMCID: PMC2819397
PMID: 19965178
url
http://doi.org/10.1109/IEMBS.2009.5335108View
Open Access

Abstract

The binding free energies of several benzamidine-like inhibitors to trypsin were examined using a polarizable potential. All the computed binding free energies are in good agreement with the experimental data. From free energy decomposition, electrostatic interaction was found to be the driving force for the binding. Structural analysis shows that the ligands form hydrogen bonds with residues and water molecules nearby in a competitive fashion. The dependence of binding free energy on molecular dipole moment and polarizability was also studied. While the binding free energy is independent on the dipole moment, it shows a negative correlation with the polarizability.

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