Dataset
Dataset for the Disposition and Toxicity of 2,2’,5,5’-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure
University of Iowa
07/23/2023
DOI: 10.25820/data.006225
Abstract
This dataset summarized a study investigating the effects of slow-release PCB 52 in female rats. This study was designed to include 4 groups of Sprague Dawley female rats (3-5 per group): Vehicle (Corn Oil), 1mg/kg PCB 52, 10mg/kg PCB 52, 100mg/kg PCB 52. In which animals were injected with their corresponding PCB 52 exposure dose via injected intraperitoneal (IP) at 5 weeks of age and euthanized after 3 weeks. The overall hypothesis was that animals exposed to a slow release of PCB 52 at different concentrations would result in gross organ differences and homeostatic disruptions in the rats.
Details
- Title: Subtitle
- Dataset for the Disposition and Toxicity of 2,2’,5,5’-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure
- Creators
- Amanda J BullertXueshu Li - University of Iowa, Occupational and Environmental HealthChunyun Zhang - University of Iowa, Occupational and Environmental HealthKendra LeeCasey F Pulliam - University of IowaAloysius J Klingelhutz - University of Iowa, Microbiology and ImmunologyLarry W Robertson - University of Iowa, Occupational and Environmental HealthHans-Joachim Lehmler - University of Iowa, Occupational and Environmental Health
- Contributors
- Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
- Resource Type
- Dataset
- DOI
- 10.25820/data.006225
- Publisher
- University of Iowa
- Grants
- Comment
- This data record was updated on 2023-08-07. A new data file was added, and the readme and data dictionary files were updated to include references and information about that data file. The added data file is combined_studies_PCB52_OPR_20230807.csv
- Date collected
- 01/31/2018–06/30/2022
- Date published
- 07/23/2023
- Date updated
- 08/07/2023
- Description methods
- Rat tissues (i.e., liver and brain) and serum were analzyed on a Q-Exactive Orbitrap mass spectrometry (Thermo Fisher Scientific, Waltham, MA, USA) with a Vanquish Flex ultra-high-performance liquid chromatograph (Thermo Fisher Scientific) with an ACQUITY UPLC-C18 column (particle size: 1.7 µM, 2.1 × 100 mm, Waters, Milford, MA, USA) at the High Resolution Mass Spectrometry Facility of the University of Iowa. Water and acetonitrile were used as mobile phase A and B, with a flow rate of 0.3 mL/min. The pressure of the chromatographic system was range from 4000 to 8000 psi. The UHPLC gradient program was as follows: starting at 5% B, held for 1 min, increased linearly to 95% B, held for 3 min, and returning to 5% B, with a hold for 4 min before the next injection. The injection volume was 2 µL. The Q-Exactive Orbitrap Mass Spectrometry was operated in the negative polarity mode. The spray volatage and current were 2472 V and 18.2 µA. The gas flow rate of sheath and auxiliary were 48 and 2 mL/min respectively. The calillary and auxiliary temperature were 256 oC and 413 oC respectively. The analyses were performed in the full scan mode with a range of 85 to 1000 m/z. The autogain control target setting was 1×106, the full scan resolution setting was 70,000 and maxum interval time (IT) was 200 ms. Data analysis The chromatographic peaks of suspected PCB metabolites were extracted from the acquired data (as .raw file) with Thermo Xcalibur (version 4.1, Thermo Fisher Scientific, USA). The chromatogram extraction was performed with a m/z tolerance of 5 ppm, mass precision decimals of 5, and the smoothing factor of 7.
- Academic Unit
- Microbiology and Immunology; Occupational and Environmental Health; Iowa Neuroscience Institute; Radiation Oncology; Humanities and Social Sciences/Scholarly Impact; Iowa Superfund Research Program
- Record Identifier
- 9984379645302771
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