Dataset
Additional file 4 of Palmitate enhances MSC immunomodulation of human macrophages via the ceramide/CCL2 axis in vitro
figshare
01/01/2025
DOI: 10.6084/m9.figshare.29849050
Abstract
Supplementary Material 4: Supplementary Figure 4: MSCs promote an M2 switch in MDMs, and this is not further enhanced by palmitate pre-exposed MSC. MSCs from 3 donors were seeded at 2.5x103 cells/well in a 24 well plate and treated with 0.4 mM palmitate for 24 hr. MSCs were then washed thoroughly twice with warm PBS and 5x103 MDMs were added in abRPMI for an MSC to MDM ratio of 1:20. The co-culture was stimulated with 100 ng/mL LPS for 24 hr and cells were harvested using a lidocaine detachment buffer. Cells were incubated with fluorochrome labelled antibodies and surface phenotype was analysed using the Attune Nxt flow cytometer. Gating was performed on live (live/dead stain, near-IR fluorescent reactive dye, Invitrogen), CD14+ (PE) cells using antibodies for CD206 (Pacific Blue), HLA-DR (FITC), CD11b (PE-Cy7), CD86 (APC), and CD163 (PerCP). Data were analysed using floreada.io. Statistical test: Ordinary one-way ANOVA with Tukey’s multiple comparisons test *p<0.05, ns; not significant. n = 3 human MDM donors.
Details
- Title: Subtitle
- Additional file 4 of Palmitate enhances MSC immunomodulation of human macrophages via the ceramide/CCL2 axis in vitro
- Creators
- Courteney Tunstead - National University of Ireland, MaynoothLaura M. Bitterlich - National University of Ireland, MaynoothJames A. Ankrum - University of IowaAndrew E. Hogan - National University of Ireland, MaynoothKaren English - National University of Ireland, Maynooth
- Resource Type
- Dataset
- DOI
- 10.6084/m9.figshare.29849050
- Publisher
- figshare
- Language
- English
- Date published
- 01/01/2025
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9985243572202771
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