Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (1-36)

T. J ROSOL; C. L STEINMEYER; L. K MCCAULEY; J. I MERRYMAN; J. R WERKMEISTER; A GRÖNE; M. T WECKMANN; D. E SWAYNE; C. C CAPEN

doi:10.1016/0165-2427(93)90042-3

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Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (1-36)

Journal article

Peer reviewed

Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (1-36)

T. J ROSOL, C. L STEINMEYER, L. K MCCAULEY, J. I MERRYMAN, J. R WERKMEISTER, A GRÖNE, M. T WECKMANN, D. E SWAYNE and C. C CAPEN

Veterinary immunology and immunopathology, Vol.35(3-4), pp.321-337

1993

DOI: 10.1016/0165-2427(93)90042-3

PMID: 8430499

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Abstract

Chicken polyclonal antibodies were prepared against a synthetic peptide corresponding to the first 36 N-terminal amino acids of parathyroid hormone-related protein (PTHrP) by immunizing laying hens. Significant increases of antibodies to PTHrP were first detected after the second immunization. Production of anti-PTHrP egg yolk antibodies peaked 1-2 weeks after the second through sixth immunizations and declined over a period of 2-4 weeks. Polyclonal IgG (IgY) to PTHrP was purified from the egg yolks with high levels of PTHrP specific binding. The anti-PTHrP IgG was used to develop a radioimmunoassay for PTHrP that was able to detect 100 pg PTHrP ml-1 (23 pM) in conditioned cell culture medium. The anti-PTHrP IgG was bound to a solid phase and utilized to immunopurify iodinated [Tyr36]-PTHrP (1-36). Anti-PTHrP IgG inhibited the in vitro biologic activity of PTHrP as demonstrated by the inhibition of adenylate cyclase stimulation in a rat osteoblast-like cell line (ROS 17/2.8). The anti PTHrP IgG was immunopurified and utilized for immunohistochemical localization of PTHrP in canine skin. Chickens were advantageous in producing large amounts of high affinity, neutralizing antibodies to a highly conserved mammalian protein such as PTHrP. The antibodies will be useful to investigate the function and metabolism of PTHrP in vivo and in vitro.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Vertebrates: endocrinology

Thyroid. Parathyroid. Ultimobranchial body

Hormones. Regulation

Details

Title: Subtitle: Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (1-36)
Creators: T. J ROSOL - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
C. L STEINMEYER - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
L. K MCCAULEY - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
J. I MERRYMAN - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
J. R WERKMEISTER - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
A GRÖNE - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
M. T WECKMANN - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
D. E SWAYNE - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
C. C CAPEN - Ohio state univ., dep. veterinary pathobiology, Columbus OH 43210, United States
Resource Type: Journal article
Publication Details: Veterinary immunology and immunopathology, Vol.35(3-4), pp.321-337
DOI: 10.1016/0165-2427(93)90042-3
PMID: 8430499
NLM abbreviation: Vet Immunol Immunopathol
ISSN: 0165-2427
eISSN: 1873-2534
Publisher: Elsevier; Amsterdam
Language: English
Date published: 1993
Academic Unit: Psychiatry; Family and Community Medicine
Record Identifier: 9984024508402771

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