Dataset
Data for: MIRO1 is required for dynamic increases in mitochondria ER contact sites and mitochondrial ATP during the cell cycle
05/29/2025
DOI: 10.25820/data.007621
Abstract
Data sets for each manuscript figure include all measurements taken.
Wound healing was assessed in mice with fibroblast-specific MIRO1 deletion. Wild-type and MIRO1-/- fibroblasts and vascular smooth muscle cells were analyzed for proliferation, cell cycle progression, MERCS number, distance, and protein composition throughout the cell cycle. The effect of restoring MIRO1 mutants was tested to assess the role of its domains. Ca2+ transients and mitochondrial metabolism were measured using biochemical, immunodetection, and fluorescence techniques.
Results showed that MERCS increased during G1/S compared to G0, accompanied by a rise in protein-protein interactions such as VDAC1-IP3R and GRP75-MIRO1 identified via proximity-ligation assays. ER/mitochondrial contacts (~40 nm) measured by split GFP also increased. Mitochondrial [Ca2+], membrane potential, and ATP levels were linked to MERCS formation during the cell cycle. MIRO1 deficiency disrupted G1/S progression, MERCS formation, ER Ca2+ release, and mitochondrial Ca2+ uptake. MIRO1 mutants lacking Ca2+-sensitive EF hands or the transmembrane domain failed to restore cell proliferation or MERCS formation.</p><p>MIRO1 regulates MERCS formation during the cell cycle, increases mitochondrial [Ca2+], and drives metabolic activity and proliferation via its EF hands.
Details
- Title: Subtitle
- Data for: MIRO1 is required for dynamic increases in mitochondria ER contact sites and mitochondrial ATP during the cell cycle
- Creators
- Benney T Endoni (Researcher) - University of Iowa, Biochemistry and Molecular BiologyOlha M Koval (Researcher) - University of Iowa, Cardiovascular MedicineChantal Allamargot (Researcher) - University of Iowa, Core Research FacilitiesTara Kortlever (Researcher) - University of IowaLan Qian (Researcher) - University of Iowa, Internal MedicineDenise Juhr (Researcher) - University of Iowa, Internal MedicineRiley J. Sadoski (Researcher) - University of IowaIsabella M Grumbach (Corresponding Author) - University of Iowa, Internal Medicine
- Contributors
- Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
- Resource Type
- Dataset
- DOI
- 10.25820/data.007621
- Grants
- R01 HL 157956 , National Heart Lung and Blood Institute (United States, Bethesda) - NHLBII01 BX000163 , United States Department of Veterans Affairs (United States, Washington) - VA17GRNT33660032, American Heart Association (United States, Dallas) - AHA22PRE902649, American Heart Association (United States, Dallas) - AHA
- Language
- English
- Date collected
- 07/31/2021–07/31/2021
- Date published
- 05/29/2025
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Humanities and Social Sciences/Scholarly Impact; Internal Medicine
- Record Identifier
- 9984815316802771
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