Dataset for Elucidating the Metabolism of Chiral PCB95 in Wildtype and Transgenic Mouse Models with Altered Cytochrome P450 Enzymes Using Intestinal Content Screening

Xueshu Li; Amanda J Bullert; Binita Gautam; Weiguo Han; Weizhu Yang; Qing-Yu Zhang; Xinxin Ding; Hans-Joachim Lehmler

doi:10.25820/data.007444

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Dataset for Elucidating the Metabolism of Chiral PCB95 in Wildtype and Transgenic Mouse Models with Altered Cytochrome P450 Enzymes Using Intestinal Content Screening

Xueshu Li, Amanda J Bullert, Binita Gautam, Weiguo Han, Weizhu Yang, Qing-Yu Zhang, Xinxin Ding and Hans-Joachim Lehmler

University of Iowa

11/18/2024

DOI: 10.25820/data.007444

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0_README_PCB95_and_metabolites_intestinal_contents19.08 kBDownload View

READMEDataset creators, funding, abstract, file list and descriptions, substance abbreviations, and methods.Open Data Commons Attribution (ODC-By) V1.0, Open Access

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D00_data dictionary6.13 kBDownload View

Data DictionaryData dictionary for tabular data files in this dataset.Open Data Commons Attribution (ODC-By) V1.0, Open Access

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D11_substance_identifiers7.88 kBDownload View

List of identifiers for the substances used in this study.Open Data Commons Attribution (ODC-By) V1.0, Open Access

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Link to Globus for downloading the data filesView

Data files and documentation are accessible via this link to Globus. Download from Globus via web browser, or Globus app.Open Data Commons Attribution (ODC-By) V1.0

Abstract

This dataset summarizes a study investigating the metabolic pathways of 2,2’,3,5,5’-pentachlorinated biphenyl (PCB 95) in the intestinal contents of wild-type (WT), Cyp2abfgs-null (KO), and CYP2A6-transgenic/Cyp2abfgs-null (KI) mice. These mice were exposed to racemic PCB 95 (1 mg/kg body weight) using stripped corn oil as the vehicle in a single gavage dose. PCB 95 and its hydroxylated metabolites (as methoxylated derivatives) were analyzed using gas chromatography-tandem mass spectrometry (GC-MS/MS) in multiple reaction monitoring (MRM) mode. Additionally, more complex PCB 95 metabolites, including various hydroxylated PCB and PCB sulfate metabolites, were analyzed using liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The dataset includes the weight of intestinal contents, mass and concentration of PCB 95 and its metabolites in the samples, and recovery rates for ongoing precision and recovery (OPR) samples as well as surrogate standards, values of enantiomeric fraction (EF) of chiral PCB95, relevant levels of untargeted PCB metabolites, and raw data of GC-MS/MS and LC-HRMS with open mzML files.

Metabolism

Metabolites

Metabolic pathways

PCB 95

PCBs

Polychlorinated biphenyls

PCB derivatives

Mice

Details

Title: Subtitle: Dataset for Elucidating the Metabolism of Chiral PCB95 in Wildtype and Transgenic Mouse Models with Altered Cytochrome P450 Enzymes Using Intestinal Content Screening
Creators: Xueshu Li - University of Iowa, Occupational and Environmental Health
Amanda J Bullert
Binita Gautam - University of Iowa, Occupational and Environmental Health
Weiguo Han - University of Arizona
Weizhu Yang - University of Arizona
Qing-Yu Zhang - University of Arizona
Xinxin Ding - University of Arizona
Hans-Joachim Lehmler - University of Iowa, Occupational and Environmental Health
Contributors: Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
Resource Type: Dataset
DOI: 10.25820/data.007444
Publisher: University of Iowa
Grants: Airborne PCBs: Sources, Exposures, Toxicities, Remediation, P42ES013661, National Institutes of Health (United States, Bethesda) - NIH
Environmental Health Sciences Research Center, P30ES005605, National Institutes of Health (United States, Bethesda) - NIH
PCB-mediated dysbiosis of the gut microbiome: A missing link in PCB-mediated neurodevelopmental disorders?, R01ES031098, National Institutes of Health (United States, Bethesda) - NIH
PCB Enantiomers Implicated in Neurodevelopmental Disorders: Identification of Individual Metabolic Factors that Determine Risk and Vulnerability, R21ES027169, National Institutes of Health (United States, Bethesda) - NIH
Molecular and Cellular Basis of PCB Developmental Neurotoxicity, R01ES014901, National Institutes of Health (United States, Bethesda) - NIH
Southwest Environmental Health Sciences Center, P30ES006694, National Institutes of Health (United States, Bethesda) - NIH
Language: English
Date collected: 09/06/2019–05/02/2020
Date published: 11/18/2024
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute; Humanities and Social Sciences/Scholarly Impact; Iowa Superfund Research Program
Record Identifier: 9984720832602771

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Dataset for Elucidating the Metabolism of Chiral PCB95 in Wildtype and Transgenic Mouse Models with Altered Cytochrome P450 Enzymes Using Intestinal Content Screening

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