Dataset for: Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signaling

Valentina Botero; Jenifer Barrios; Anneke Knauss; Greta Dahlen; Ethan Rosendahl; Kenneth J Colodner; Seth M Tomchik

doi:10.25820/data.007850

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Dataset for: Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signaling

Valentina Botero, Jenifer Barrios, Anneke Knauss, Greta Dahlen, Ethan Rosendahl, Kenneth J Colodner and Seth M Tomchik

University of Iowa

02/17/2026

DOI: 10.25820/data.007850

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Abstract

Effects of loss of function manipulations in the Nf1 gene (genomic mutations or RNAi-mediated knockdown). These were combined with knockdown of various downstream signaling molecules in the MAPK signaling pathway (MEK, ERK), Akt/mTOR (Akt, Raptor, 4E-BP, S6K), and cAMP/PKA. Several assays were performed, including metabolic analysis (CO2 respirometry) and behavioral analysis (grooming quantification), biochemistry (western blots), analysis of light and transmission electron microscopy images, and morphological analysis of mitochondria. Data are organized by figure panels in a submitted manuscript by Botero et al., and described in the readme file.

Behavioral Biology

Disease

Metabolism

Neuroscience

Signaling

MAPK

mTOR

Grooming

Details

Title: Subtitle: Dataset for: Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signaling
Creators: Valentina Botero (Author) - University of Iowa, Neuroscience and Pharmacology
Jenifer Barrios (Author) - University of Iowa
Anneke Knauss - University of Iowa, Neuroscience and Pharmacology
Greta Dahlen - University of Iowa, Neuroscience and Pharmacology
Ethan Rosendahl (Author)
Kenneth J Colodner (Author) - Mount Holyoke College
Seth M Tomchik (Corresponding Author) - University of Iowa, Iowa Neuroscience Institute
Contributors: Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
Resource Type: Dataset
DOI: 10.25820/data.007850
Publisher: University of Iowa
Grants: Dopaminergic circuit modulation of learning and arousal-mediated memory enhancement, R01NS114403, National Institute of Neurological Disorders and Stroke (United States, Bethesda) - NINDS
Mechanisms of Nf1 pathophysiology underlying hyperactivity, R01NS097237, National Institute of Neurological Disorders and Stroke (United States, Bethesda) - NINDS
The role of Nf1 in sleep-dependent regulation of metabolic function, R21NS124198, National Institute of Neurological Disorders and Stroke (United States, Bethesda) - NINDS
Mechanisms of Neuronal Dysregulation Underlying Behavioral Alterations in Neurofibromatosis Type 1, NF230039, Congressionally Directed Medical Research Programs (United States, Frederick) - CDMRP
Mechanisms of compartmentalized plasticity in learning and memory, R01NS124716, National Institute of Neurological Disorders and Stroke (United States, Bethesda) - NINDS
Genetic and molecular mechanisms of Nf1-dependent neuronal regulation of metabolism, R01NS126361, National Institute of Neurological Disorders and Stroke (United States, Bethesda) - NINDS
Language: English
Date collected: 07/08/2021–12/30/2025
Date published: 02/17/2026
Academic Unit: Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Humanities and Social Sciences/Scholarly Impact; Neuroscience and Pharmacology
Record Identifier: 9984847540402771

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