Dataset
Metabolomics Dataset for The Effects of Benoxacor on the Liver and Gut Microbiome of C57BL/6 Mice
University of Iowa
12/02/2021
DOI: 10.25820/data.006157
Abstract
This dataset summarizes the data from the targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics analysis of serum and liver tissue from thirty-two four-week-old male C57BL/6J mice exposed between 9 and 11 A.M. to corn oil (p.o., 10 ml/kg b.w.) or to benoxacor in corn oil for a final dose (p.o., 0.5 mg/kg b.w., 5 mg/kg b.w., or 50 mg/kg b.w.).
Details
- Title: Subtitle
- Metabolomics Dataset for The Effects of Benoxacor on the Liver and Gut Microbiome of C57BL/6 Mice
- Creators
- Derek SimonsenHans-Joachim Lehmler - University of Iowa, Occupational and Environmental Health
- Resource Type
- Dataset
- DOI
- 10.25820/data.006157
- Publisher
- University of Iowa
- Grants
- Language
- English
- Date collected
- 07/28/2020
- Date published
- 12/02/2021
- Description methods
- Description of methods used for collection/generation of data: Mice were exposed for three consecutive days between 9 and 11 A.M. to corn oil (p.o., 10 ml/kg b.w.) or to benoxacor in corn oil for a final dose (p.o., 0.5 mg/kg b.w., 5 mg/kg b.w., or 50 mg/kg b.w.). Each exposure group (0, 0.5, 5, and 50 mg/kg b.w. benoxacor) consisted of 8 mice (2 cages of 4 animals/cage). Animals were euthanized 24 h after the final dose by carbon dioxide (CO2) asphyxiation, followed by the harvest of vital tissues. The IACUC of the University of Iowa approved all methods (protocol number: 0082057-001). Whole blood was obtained via cardiac puncture, immediately transferred to a MiniCollect serum separator (Greiner Bio-One, Kremsmunster, Austria), and kept on ice for a minimum of 30 minutes to allow the blood to clot. The serum was collected through centrifugation at 1503 g at 4 øC for 20 minutes. Serum was transferred to 1.5 mL plastic tubes (Eppendorf North America, Canada) and stored at -80 øC until further analysis. Liver tissue was excised and flash-frozen in liquid nitrogen for metabolomics measurements. All liver samples were stored at -80 øC until analysis. Targeted metabolomic data were collected with a screening list containing 361 metabolites following protein precipitation with methanol at the Northwest Metabolomics Research Center (University of Washington, Seattle, Washington) in the mouse liver and serum. Briefly, samples were extracted and analyzed in the multiple-reaction-monitoring (MRM) mode on XBridge BEH Amide XP columns (Waters Technologies Corporation, Milford, MA, USA) with an AB Sciex 6500+ Triple Quadrupole MS equipped with ESI ionization source in the ESI- and ESI+ ionization mode. In liver samples, 172 metabolites were measured, while 173 metabolites were quantified in the plasma samples. Generated MS data for the tissue samples were normalized versus BCA total protein count. The MS data for the plasma samples were not normalized. Describe any quality-assurance procedures performed on the data: A pooled human serum sample was used to monitor system performance, and pooled study samples were used to monitor data reproducibility. Both sample batches (32 tissue and 32 plasma samples) generated reproducible data with an average coefficient of variances (CVs) of 5.6 to 6.9%.
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute
- Record Identifier
- 9984186056402771
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