Dataset
Quantitative microscopy data to determine the effect of sequence divergence between orthologous transcription factor proteins on their nuclear localization behavior
University of Iowa
02/19/2025
DOI: 10.25820/data.007484
Abstract
Combinatorial control by transcription factors (TFs) is central to eukaryotic gene regulation, yet its mechanism, evolution, and regulatory impact are not well understood. Here we use natural variation in the yeast phosphate starvation (PHO) response to examine the genetic basis and species variation in TF interdependence. In Saccharomyces cerevisiae, main TF Pho4 relies on the co-TF Pho2 to regulate ~28 genes, whereas in the related pathogen Candida glabrata, Pho4 has reduced Pho2 dependence and regulates ~70 genes. We found C. glabrata Pho4 (CgPho4) binds the same motif with 3-4 fold higher affinity. Machine learning and yeast one-hybrid assay identify two intrinsically disordered regions (IDRs) in CgPho4 that boost its activation domain’s activity. In ScPho4, an IDR next to the DNA binding domain both allows for enhanced activity with Pho2 and inhibits activity without Pho2. This study reveals how IDR divergence drives TF interdependence evolution by influencing activation potential and autoinhibition.
Details
- Title: Subtitle
- Quantitative microscopy data to determine the effect of sequence divergence between orthologous transcription factor proteins on their nuclear localization behavior
- Creators
- Bin Z He - University of Iowa, Biology
- Contributors
- Brian Westra (Contributor) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
- Resource Type
- Dataset
- Publisher
- University of Iowa
- DOI
- 10.25820/data.007484
- Language
- English
- Date published
- 02/19/2025
- Academic Unit
- Biology; Humanities and Social Sciences/Scholarly Impact
- Record Identifier
- 9984786857502771
Metrics
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