Dataset
Repetitive hypoxic preconditioning protects retinal ganglion cell complex thickness, retinal ganglion cell pattern electroretinogram function, and retinal ganglion cell survival against damage in male C57BL/6J mice caused by exposure to blast
Open Data Commons for Traumatic Brain Injury (ODC-TBI)
2026
DOI: 10.34945/f53k6n
Abstract
STUDY PURPOSE: The purpose of this study was to determine if repetitive hypoxic preconditioning (HPC) can prevent blast-mediated traumatic brain injury (bTBI)-mediated retinal ganglion cell (RGC) damage and death. DATA COLLECTED: Male C57BL/6J mice were subjected to bTBI using an advanced blast simulator to deliver a blast wave with the following characteristics: 31.5 PSI positive peak pressure with a 6.15 ms
postive duration, -4.99 PSI peak negative pressure with a 7.76 ms duration. The overall positive impulse (area under the curve) was 63.02 PSI, and the overall negative impulse was -20.59 PSI.
Three groups of mice with 15 mice per group were analyzed including 1) Sham mice not exposed to blast injury 2) Mice exposed to bTBI 3) Mice exposed to six sessions of hypoxic preconditioning for 1 hour at 11% oxygen beginning two weeks prior to bTBI (bTBI + HPC).
Four-weeks following exposure to bTBI or sham, functional and structural analysis of RGCs was performed.
The pattern electroretinogram (PERG) analyzed the function of the RGCs by evaluating the amplitude of the wave form.
Optical coherence tomography (OCT) evaluated the combined thickness of the RGC complex, which included the retinal nerve fiber layer, Inner plexiform layer, and ganglion cell layer.
BRN3A antibody labeling was subsequently used to quantify the number of RGCs surviving at the termination of the study. RGC survival was calculated using retinal flat mounts. Images were obtained in all mice from the central, mid-peripheral, and peripheral locations.
HPC37 was removed from the study due to pre-defined animal health criteria. DATA USAGE NOTES:
Details
- Title: Subtitle
- Repetitive hypoxic preconditioning protects retinal ganglion cell complex thickness, retinal ganglion cell pattern electroretinogram function, and retinal ganglion cell survival against damage in male C57BL/6J mice caused by exposure to blast
- Creators
- Matthew Harper - University of Iowa
- Resource Type
- Dataset
- DOI
- 10.34945/f53k6n
- Publisher
- Open Data Commons for Traumatic Brain Injury (ODC-TBI)
- Grant note
- Department of Veterans Affairs (1 I01 RX003389 (MMH)) Department of Veterans Affairs (1 I01 BX005855 (MMH))
- Language
- English
- Date published
- 2026
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9985164610702771
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