Dissertation
Wound microbiome in acute open wounds
University of Iowa
Doctor of Philosophy (PhD), University of Iowa
Summer 2024
DOI: 10.25820/etd.007628
Abstract
This dissertation explores the role of the wound microbiome in acute open wounds, structured into three distinct studies. The first study presents a comprehensive scoping review of the literature on Deep Sternal Wound Infection (DSWI), focusing on dimensions of the wound microbiome: microbial load, microbial diversity, presence/abundance of common wound microorganisms, and biofilm formation. Findings suggest a significant reliance on culture techniques and the need for advanced molecular methods to better understand the wound microbiome associated with DSWI. The second study investigated opioid tolerance in acute open wounds, comparing patient and wound factors, wound microbiome, and inflammatory mediators between opioid-tolerant and non-tolerant patients. Results indicate significant predictors, including younger age, higher levels of depression and resting wound pain, and reduced bacterial richness, underscoring the necessity for targeted interventions to manage pain and reduce opioid dependency in patients with acute open wounds. The third study examines the microbiome and inflammatory mediator expression in abdominal surgical wounds, differentiating between infected and non-infected cases. It identifies a higher abundance of anaerobes in infected wounds and links specific inflammatory mediators like ARG1 and MMP-8 to abdominal surgical site infections. This dissertation highlights the importance of using advanced molecular techniques and suggests future research directions to develop interventions that can modify the impact of the microbiome on wound pathogenesis and healing, potentially reducing the incidence and severity of both SSIs and opioid tolerance.
Details
- Title: Subtitle
- Wound microbiome in acute open wounds
- Creators
- Jaewon Bae
- Contributors
- Sue Gardner (Advisor)Elizabeth Grice (Committee Member)Anna Krupp (Committee Member)Youngeun Cho (Committee Member)
- Resource Type
- Dissertation
- Degree Awarded
- Doctor of Philosophy (PhD), University of Iowa
- Degree in
- Nursing
- Date degree season
- Summer 2024
- Publisher
- University of Iowa
- DOI
- 10.25820/etd.007628
- Number of pages
- viii, 154 pages
- Copyright
- Copyright 2024 Jaewon Bae
- Grant note
- For this study, a secondary data analysis was conducted from a larger study (PI: Gardner), which was funded by the National Institute of Nursing Research (R01NR015642). (66)
- Language
- English
- Date submitted
- 07/05/2024
- Description illustrations
- illustrations, graphs, tables
- Description bibliographic
- Includes bibliographical references (pages 97-139).
- Public Abstract (ETD)
- This dissertation explore the microscopic world of bacteria living in acute open wounds, aiming to understand how they affect healing and infection. The research is divided into three studies. The first study reviews literature on Deep Sternal Wound Infection (DSWI), examining aspects like the amount and diversity of bacteria, the presence of common harmful bacteria, and biofilm formation. It highlights the need for better molecular methods to study these bacteria accurately. The second study investigates how opioid use for pain relief can be related to various factors in patients with acute open wounds. It finds that younger patients, those with higher levels of depression and pain at rest, and those with fewer types of bacteria in their wounds are more likely to be opioid-tolerant. This suggests a need for better pain management strategies targeting those factors to reduce opioid dependency. The third study focuses on abdominal surgery wounds, comparing infected and non-infected wounds. It finds that infected wounds have more anaerobic bacteria and identifies higher release of specific inflammatory substances like ARG1 and MMP-8 linked to these infections. Overall, this dissertation highlights the importance of using advanced molecular techniques and suggests future research to improve wound care, aiming to reduce infections and opioid use disorders in patients with acute open wounds.
- Academic Unit
- Nursing
- Record Identifier
- 9984697846702771
Metrics
2 Record Views