Thesis
Development of In vitro and In vivo systems to minimize scarring
University of Iowa
Master of Science (MS), University of Iowa
Autumn 2021
DOI: 10.17077/etd.006239
Abstract
The wound healing process is consists of three continuous, overlapping, and precisely programmed phases that must occur in the proper sequence, at the proper time, and for the proper duration to have the desired outcome [1]. Impaired healing is multifactorial and is a result of an uncoordinated healing process. Treating pathological healing situations have a significant clinical and financial burden on healthcare systems worldwide [2]. Understanding the mechanical and biochemical mechanisms that influence wound healing can aid in discovering treatment methods for pathological tissue repair. It has been found that the process of wound healing is greatly influenced by the balance of intracellular and extracellular forces [3, 4]. Dysregulation of fibroblast activity is believed to be one of the main contributors leading to imbalance and tissue dysfunction [3]. Through the development of in vitro and in vivo models, we can identify the mechanical and biochemical stimuli that contribute to aberrant fibroblast activity leading to fibrosis. Additionally, these models allow us to investigate the potential of different treatment strategies, including the actomyosin inhibitor, blebbistatin, to alter fibroblast behavior and reduce fibrotic activity. With specific aims for clinical translation, development of these models is essential in providing the foundation to combat pathological wound healing.
Details
- Title: Subtitle
- Development of In vitro and In vivo systems to minimize scarring
- Creators
- Jordan Jensen
- Contributors
- Edward Sander (Advisor)James Ankrum (Committee Member)James Martin (Committee Member)
- Resource Type
- Thesis
- Degree Awarded
- Master of Science (MS), University of Iowa
- Degree in
- Biomedical Engineering
- Date degree season
- Autumn 2021
- DOI
- 10.17077/etd.006239
- Publisher
- University of Iowa
- Number of pages
- x, 84 pages
- Copyright
- Copyright 2021 Jordan Jensen
- Language
- English
- Description illustrations
- color illustrations
- Description bibliographic
- Includes bibliographical references (pages 82-84)
- Public Abstract (ETD)
The ‘holy grail’ of wound healing is ‘scarless wound healing’. Unfortunately, that is rarely the case. Scarring and pathological wound healing states have a significant clinical and financial burden on the United States’ healthcare system. The wound healing process encompasses everything from a scratch on the surface of the skin to significant trauma to a joint. Three precisely programmed, overlapping phases must interact seamlessly to result in ‘normal’ healing, which still results in a scar. In any of those phases, there are several factors that can influence the outcome of this delicate process.
Developing an understanding of the mechanisms at work is the first step in preventing pathological healing. By simplifying the overall process and identifying cell responses to different mechanical and biochemical stimuli, we can identify what factors are contributing to overactive healing that leads to scarring. This simplification allows us to experiment with different therapies, and eventually translate to more clinically relevant scenarios including animal models. The development of these models can help to gain better understanding of wound healing and help reduce the burden of scarring.
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center
- Record Identifier
- 9984210943302771
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