Thesis
Evaluation of the effect of fluoride topical agents on the prevention of demineralization adjacent to glass ionomer restoration In vitro
University of Iowa
Master of Science (MS), University of Iowa
Autumn 2021
DOI: 10.17077/etd.006238
Abstract
Objectives: This study aims to determine whether the silver diamine fluoride agent (SDF) effectively prevents secondary caries on glass ionomer type of restorations as compared to sodium fluoride (NaF) varnish.Methods: A total of 45 molar teeth with non-carious roots were prepared for a class V cavity lesion (4x2x2 mm). The specimens were allocated to 9 different groups: group 1, Fuji II LC® restoration; group 2, Fuji II LC® restoration followed by 38% SDF; group 3, Fuji II LC® restoration followed by 5% NaF varnish; group 4, Equia Forte® restoration; group 5, Equia Forte® restoration followed by 38% SDF; group 6, Equia Forte® followed by 5% NaF varnish; group 7, Equia Forte® restoration with resin coating; group 8, Equia Forte® restoration with resin coating followed by 38% SDF; group 9, Equia Forte® with resin coating followed by 5% NaF varnish. Samples were immersed in S. mutans and L. casei in MRS medium + 5% sucrose to develop a biofilm. Subsequently, the MRS medium was replenished daily and incubated at 37°C for 8 days. Polarized light microscope was used to measure demineralization. The deepest point of the lesion adjacent to the restoration of the tooth surface was measured from the images captured by the polarized light microscope. With demineralization depth as the outcome measure, Kruskal-Wallis test was used to compare the median depth measurements between 10 different groups. Dunn tests were used for pairwise comparison.
Results: There were no statistically significant differences between the measurements for the three restorative materials. The lesion depth was less in restorations with SDF and NaF than those without SDF and NaF agents.
Conclusions: The SDF and NaF could provide long-term antibacterial activity and potentially extend the longevity of the restorations and avoid repair or replacement of restorations.
Details
- Title: Subtitle
- Evaluation of the effect of fluoride topical agents on the prevention of demineralization adjacent to glass ionomer restoration In vitro
- Creators
- Yun Jung Kim
- Contributors
- Justine Kolker (Advisor)Jeffrey Banas (Committee Member)Steve Armstrong (Committee Member)Leonardo Marchini (Committee Member)Erliang Zeng (Committee Member)
- Resource Type
- Thesis
- Degree Awarded
- Master of Science (MS), University of Iowa
- Degree in
- Oral Science
- Date degree season
- Autumn 2021
- DOI
- 10.17077/etd.006238
- Publisher
- University of Iowa
- Number of pages
- xii, 108 pages
- Copyright
- Copyright 2021 Yun Jung Kim
- Language
- English
- Description illustrations
- color illustrations
- Description bibliographic
- Includes bibliographical references (pages 101-108)
- Public Abstract (ETD)
The purpose of this experiment is to test the effectiveness of silver diaminefluoride (SDF) and sodium fluoride (NaF) in preventing secondary root caries.
A total of 45 caries free molar teeth were used. The samples were placed into 9 different groups: group 1, Fuji II LC® restoration; group 2, Fuji II LC® restoration then applied with 38% SDF; group 3, Fuji II LC® restoration then applied with 5% NaF varnish; group 4, Equia Forte® restoration; group 5, Equia Forte® restoration then applied with 38% SDF; group 6, Equia Forte® followed by 5% NaF varnish; group 7, Equia Forte® restoration with resin coating; group 8, Equia Forte® restoration with resin coating then applied with 38% SDF; group 9, Equia Forte® with resin coating then applied with 5% NaF varnish. Samples were subjected to S. mutans and L. casei bacterial challenge for 8 days. Polarized light microscope was used to measure demineralization. The lesion depth was assessed and measured using statical analysis to determine if there is any significant difference in mean lesion depth among different treatment groups.
The lesion depth was less in restorations with SDF and NaF than those without SDF and NaF agents. The application SDF and NaF agents is recommended to extend the longevity of the restorations.
- Academic Unit
- Oral Pathology, Radiology and Medicine
- Record Identifier
- 9984210641202771
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