Thesis
The epigenetic studies in peri-implantitis subjects
University of Iowa
Master of Science (MS), University of Iowa
Summer 2022
DOI: 10.25820/etd.006830
Abstract
Aim: To investigate, using whole-genome bisulfite sequencing (WGBS), the differential methylation changes in the tissue samples obtained from peri-implantitis subjects compared to the tissue samples from healthy subjects.
Materials and Methods: Systemically healthy subjects (n = 5) with a diagnosis of periodontal health undergoing functional or esthetic crown lengthening and systemically healthy subjects (n = 5) with the diagnosis of peri-implantitis requiring surgical management of peri-implantitis were recruited from the University of Iowa, College of Dentistry Department of Periodontics. The tissue samples were stored in AllProtect Tissue Reagent (Qiagen) and stored in -80°C freezer until DNA extraction. DNA was extracted from each tissue biopsy using QiAmp Mini Spin Column (Qiagen), and the purity was ensured through agarose gel electrophoresis and the concentration was measured using Qubit dsDNA kit (Invitrogen). WGBS and statistical analyses to determine the differential methylation levels between peri-implantitis and control samples were performed at Novogene (Novogene Corporation Inc.)
Results: We identified CpG island methylation levels from the level of the entire genome down to the level of KEGG pathways. These methylation levels were compared between the healthy control samples and peri-implantitis samples to identify potential differences. Both hyper- and hypomethylation differences were observed at the chromosomal and KEGG pathway levels, respectively. The following 11 differentially methylated KEGG pathways were identified: autophagy, axon guidance, HPV pathway, MAPK pathway, metabolic pathway, mitophagy, pathways in cancer, PI3k-Akt signaling pathway, proteoglycans in cancer, Rap1 signaling, and Ras signaling.
Conclusion: Within the limitations of this pilot study, we have identified Cytosine-phosphate-Guanine (CpG) islands that are differently methylated between age and gender-matched samples from control and peri-implantitis subjects. These differentially methylated regions are associated with KEGG pathways that our data suggests may play a role in the pathogenesis of peri-implantitis and warrant further investigation in a larger sample size.
Details
- Title: Subtitle
- The epigenetic studies in peri-implantitis subjects
- Creators
- Richard Sinn
- Contributors
- Satheesh Elangovan (Advisor)Shankar Rengasamy Venugopalan (Advisor)Sukirth Ganesan (Committee Member)Erliang Zeng (Committee Member)
- Resource Type
- Thesis
- Degree Awarded
- Master of Science (MS), University of Iowa
- Degree in
- Oral Science
- Date degree season
- Summer 2022
- DOI
- 10.25820/etd.006830
- Publisher
- University of Iowa
- Number of pages
- viii, 82 pages
- Copyright
- Copyright 2022 Richard Sinn
- Grant note
- Funding from the International Team of Implantology (ITI) Foundation
- Language
- English
- Description illustrations
- illustrations
- Description bibliographic
- Includes bibliographical references (pages 68-82).
- Public Abstract (ETD)
One of the hallmark signs of periodontitis is bone loss around the tooth, and it is one of the most prevalent diseases throughout the world. In the United States, almost half of adults over the age of 30 have periodontal disease. If an individual does not receive treatment, they will likely experience tooth loss. When someone loses a tooth, today’s gold standard for replacing it is a dental implant. Just like bone loss around teeth, bone around implants could undergo resorption, which is called peri-implantitis. Currently, there is no single clinical strategy that is utilized to predictably treat peri-implantitis. While we know there are some similarities between the development of periodontitis and peri-implantitis, there is a lot we still do not understand.
Therefore, the aim of this study is to analyze the DNA from tissues around the teeth of periodontally healthy individuals and compare it to the DNA from tissues around an implant with peri-implantitis. By analyzing the differences between the DNA, we attempted to determine the etiology of peri-implantitis. If we can better understand what causes peri-implantitis we may be able to use that information to create a more predictable way to treat this condition.
- Academic Unit
- Oral Pathology, Radiology and Medicine
- Record Identifier
- 9984285152302771
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