Journal article
α-Dystroglycan can mediate arenavirus infection in the absence of β-dystroglycan
Virology (New York, N.Y.), Vol.316(2), pp.213-220
2003
DOI: 10.1016/j.virol.2003.07.002
PMID: 14644604
Abstract
Dystroglycan (DG) is a highly versatile cell surface molecule that provides a molecular link between the extracellular matrix (ECM) and the actin-based cytoskeleton. Encoded by a single gene, DG is posttranslationally processed to form α-DG, a peripheral protein identified as the cellular receptor for lymphocytic choriomeningitis virus (LCMV) and Lassa fever virus (LFV), and the membrane-spanning subunit β-DG. The link of β-DG to the actin-based cytoskeleton and its association with the cellular signal transduction network suggest that it may function as an essential cofactor for the activity of α-DG as a virus receptor. To address this issue, we constructed a deletion mutant lacking the cytoplasmic domain of β-DG and a C-terminal fusion between α-DG and the transmembrane domain of PDGF receptor. Both mutants were functional as virus receptors, indicating that β-DG does not act as a cofactor with α-DG for arenavirus binding and entry. These observations are in agreement with the fact that LCMV infection is independent from the structural integrity of the actin-based cytoskeleton and suggest that α-DG functions primarily in the attachment of arenaviruses to the cell surface.
Details
- Title: Subtitle
- α-Dystroglycan can mediate arenavirus infection in the absence of β-dystroglycan
- Creators
- Stefan Kunz - The Scripps Research Institute, Division of Virology, Department of Neuropharmacology, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USAKevin P Campbell - Howard Hughes Medical Institute, University of Iowa College of Medicine, Department of Physiology and Biophysics, Neurology, Iowa City, IA 52242, USAMichael B.A Oldstone - The Scripps Research Institute, Division of Virology, Department of Neuropharmacology, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.316(2), pp.213-220
- DOI
- 10.1016/j.virol.2003.07.002
- PMID
- 14644604
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2003
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068377602771
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